(A) Depiction of genome-scale CRISPR knockout (GeCKO) screen workflow. After lentiviral transduction of the sgRNA library, transduced cells were selected by puromycin. Cells were then challenged with SVV to select for resistant cells. (B) The screen identified ANTXR1 (blue) and TEX2 (red) as the most significant hits. Nontargeting control sgRNAs are highlighted in black. Log fold change (logFC) in selected over control pools is indicated on the vertical axis as a function of the average log counts per million reads (logCPM). (C) HAP1 cells were transduced with individual sgRNAs identified from the HAP1 GeCKO screen. Cell viability was assayed in the absence (light gray) or presence (black) of SVV. Each bar corresponds to the average of n = 6 replicates with error bars representing SD. Dashed lines indicate parental HAP1 cell viability in the absence and presence of SVV. (D) Individual sgRNAs identified in the 25 H446 GeCKO screen colonies were transduced into parental H446 cells. Cell viability was tested in the absence (light gray) or presence (black) of SVV. Parental H446 cell viability in the absence and presence of SVV is indicated with dashed lines. Each bar corresponds to the average of n = 6 replicates with error bars representing SD.