(A) Representative cross-sectional images of the glial lamina region of the optic nerve from control CxWT animals immunostained for GFAP, SMI32, and DAPI. Magnified images of the area outlined in the left panel show the immunolabeling pattern for GFAP and SMI32. Scale bars: 100 μm for all panels in the left column and 25 μm for panels in all other columns. (B) Representative cross-sectional images of the glial lamina region of the optic nerve from CxWT animals 8 weeks after initial microbead injection. Conventions are the same as in A. (C) Representative cross-sectional images of the glial lamina region of the optic nerve from Cx36^–/– mice under control conditions. Conventions are the same as in A. (D) Representative cross-sectional images of the glial lamina region of the optic nerve from Cx36^–/– animals 8 weeks after initial microbead injection. Conventions are the same as in A. (E) Quantification of GFAP labeling in the cross sections of optic nerves of CxWT mice under control and glaucomatous conditions (n = 5 optic nerves per group). (F) Quantification of GFAP labeling in the cross sections of optic nerves of Cx36^–/– mice under control and glaucomatous conditions (n = 5 optic nerves per group). (G) Quantification of SMI32 labeling in the cross sections of optic nerves of CxWT mice under control and glaucomatous conditions (n = 5 optic nerves per group). (H) Quantification of SMI32 labeling in the cross sections of Cx36^–/– mice under control and glaucomatous conditions (n = 5 optic nerves per group). Z-stack: 5 sections, 0.7-μm steps for all images. Data are presented as mean ± SEM. *P < 0.05, ***P < 0.001, Student’s t test.