(A) DC numbers in unfractionated skin or indicated skin fraction as assessed by flow cytometry. Numbers are reported per 8-mm punch. n = 5–7 mice per condition over 3 to 4 experiments. (B and C) Back skin from WT mice or zDC^GFP/GFP→WT chimeras (zDC^GFP/GFP chimera) was stained for GFP (red) and DAPI (blue). Representative of n = 2 mice per condition. (B) Representative images. Dashed line indicates division between dermis and DWAT. (C) GFP⁺ cell numbers (left) and GFP⁺ cells per area (right) in the dermis and DWAT of zDC^GFP/GFP chimeras. Each symbol represents a field; similarly shaded symbols are from the same chimera. (D) DC numbers per punch as assessed by flow cytometry in WT mice treated with PBS or BLM as indicated. n = 4–8 over 2 to 4 experiments. (E and F) zDC^GFP/GFP chimeras were treated with BLM for 21 days, and then back skin was stained for GFP (red) and DAPI (blue). n = 2 chimeras. (E) Representative image. Dashed line indicates division between dermis and DWAT. (F) GFP⁺ cell numbers (left) and GFP⁺ cells per area (right). Each symbol represents a field; similarly shaded symbols are from the same chimera. HPF, high-power field. (G and H) Human systemic sclerosis (SSc) lesional skin sections stained for CD1c (red), perilipin (white), and DAPI (blue). n = 3 individuals. (G) Representative images. Orange particles are autofluorescent and nonspecific. Insets highlight nucleated CD1c⁺ cells. (H) CD1c⁺ cells per area. Each symbol represents a field; similarly shaded symbols are from the same patient. Scale bars: 20 μm. *P < 0.05, **P < 0.01, ***P < 0.001 using 2-tailed unpaired Student’s t test. Error bars depict the SD in D and the SEM in other graphs.