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Research Article Free access | 10.1172/JCI806

Alpha2-adrenergic receptor-mediated release of lysophosphatidic acid by adipocytes. A paracrine signal for preadipocyte growth.

P Valet, C Pagès, O Jeanneton, D Daviaud, P Barbe, M Record, J S Saulnier-Blache, and M Lafontan

Institut National de la Recherche Médicale (INSERM U317), Institut Louis Bugnard, Université Paul Sabatier, CHR Rangueil, 31403 Toulouse Cedex 4, France.

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Institut National de la Recherche Médicale (INSERM U317), Institut Louis Bugnard, Université Paul Sabatier, CHR Rangueil, 31403 Toulouse Cedex 4, France.

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Institut National de la Recherche Médicale (INSERM U317), Institut Louis Bugnard, Université Paul Sabatier, CHR Rangueil, 31403 Toulouse Cedex 4, France.

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Institut National de la Recherche Médicale (INSERM U317), Institut Louis Bugnard, Université Paul Sabatier, CHR Rangueil, 31403 Toulouse Cedex 4, France.

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Institut National de la Recherche Médicale (INSERM U317), Institut Louis Bugnard, Université Paul Sabatier, CHR Rangueil, 31403 Toulouse Cedex 4, France.

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Institut National de la Recherche Médicale (INSERM U317), Institut Louis Bugnard, Université Paul Sabatier, CHR Rangueil, 31403 Toulouse Cedex 4, France.

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Institut National de la Recherche Médicale (INSERM U317), Institut Louis Bugnard, Université Paul Sabatier, CHR Rangueil, 31403 Toulouse Cedex 4, France.

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Institut National de la Recherche Médicale (INSERM U317), Institut Louis Bugnard, Université Paul Sabatier, CHR Rangueil, 31403 Toulouse Cedex 4, France.

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Published April 1, 1998 - More info

Published in Volume 101, Issue 7 on April 1, 1998
J Clin Invest. 1998;101(7):1431–1438. https://doi.org/10.1172/JCI806.
© 1998 The American Society for Clinical Investigation
Published April 1, 1998 - Version history
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Abstract

In the search for the existence of adrenergic regulation of the autocrine/paracrine function of the white adipose tissue, it was observed that conditioned media from isolated adipocytes or dialysates obtained by in situ microdialysis of human subcutaneous adipose tissue increased spreading and proliferation of 3T3F442A preadipocytes. These effects were amplified when an alpha2-adrenergic agonist was present during the obtention of conditioned media and microdialysates. This alpha2-adrenergic-dependent trophic activity was completely abolished by pretreatment of the conditioned media or microdialysates with the lysophospholipase, phospholipase B. Among the different lysophospholipids tested only lysophosphatidic acid (LPA) was able to induce spreading and proliferation of 3T3F442A preadipocytes. Moreover, previous chronic treatment of 3T3F442A preadipocytes with LPA which led to a specific desensitization of LPA responsiveness, abolished the alpha2-adrenergic-dependent trophic activities of the conditioned media and microdialysates. Finally, alpha2-adrenergic stimulation led to a rapid, sustained, and pertussis toxin-dependent release of [32P]LPA from [32P]-labeled adipocytes. Based upon these results it was proposed that in vitro and in situ stimulation of adipocyte alpha2-adrenergic receptors provokes the extracellular release of LPA leading, in turn, to regulation of preadipocyte growth.

Version history
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