Natriuretic peptides enhance the oxidative capacity of human skeletal muscle
Stefan Engeli, … , Jens Jordan, Cedric Moro
Stefan Engeli, … , Jens Jordan, Cedric Moro
Published November 1, 2012
Citation Information: J Clin Invest. 2012;122(12):4675-4679. https://doi.org/10.1172/JCI64526.
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Brief Report Muscle biology

Natriuretic peptides enhance the oxidative capacity of human skeletal muscle

Abstract

Cardiac natriuretic peptides (NP) are major activators of human fat cell lipolysis and have recently been shown to control brown fat thermogenesis. Here, we investigated the physiological role of NP on the oxidative metabolism of human skeletal muscle. NP receptor type A (NPRA) gene expression was positively correlated to mRNA levels of PPARγ coactivator-1α (PGC1A) and several oxidative phosphorylation (OXPHOS) genes in human skeletal muscle. Further, the expression of NPRA, PGC1A, and OXPHOS genes was coordinately upregulated in response to aerobic exercise training in human skeletal muscle. In human myotubes, NP induced PGC-1α and mitochondrial OXPHOS gene expression in a cyclic GMP–dependent manner. NP treatment increased OXPHOS protein expression, fat oxidation, and maximal respiration independent of substantial changes in mitochondrial proliferation and mass. Treatment of myotubes with NP recapitulated the effect of exercise training on muscle fat oxidative capacity in vivo. Collectively, these data show that activation of NP signaling in human skeletal muscle enhances mitochondrial oxidative metabolism and fat oxidation. We propose that NP could contribute to exercise training–induced improvement in skeletal muscle fat oxidative capacity in humans.

Authors

Stefan Engeli, Andreas L. Birkenfeld, Pierre-Marie Badin, Virginie Bourlier, Katie Louche, Nathalie Viguerie, Claire Thalamas, Emilie Montastier, Dominique Larrouy, Isabelle Harant, Isabelle de Glisezinski, Stefanie Lieske, Julia Reinke, Bibiana Beckmann, Dominique Langin, Jens Jordan, Cedric Moro

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Figure 3

NP increases mitochondrial oxidative metabolism in human primary myotubes.

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NP increases mitochondrial oxidative metabolism in human primary myotube...
(A) Representative blots of PGC-1α and OXPHOS proteins and (B) quantitative bar graphs of PGC-1α protein and (C) OXPHOS proteins after 72-hour treatment with 100 and 10 nM ANP, respectively (n = 6). C, control. (D) mtDNA content, (E) mass, and (F) palmitate oxidation were measured after 72-hour treatment with 10 nM ANP (n = 4–6). (G) Oxygen consumption rate (OCR) after 72-hour treatment with 10 nM ANP or vehicle (control) at baseline and after injection of oligomycin (ATP synthase inhibitor), FCCP (uncoupling agent), and rotenone (complex I inhibitor) plus myxothiazol (complex III inhibitor) (n = 8 experiments; control columns, mean ± SEM of 75 wells; ANP columns, mean ± SEM of 72 wells). (H) Changes in fatty acid transport genes in response to 72-hour treatment with 10 nM ANP (n = 6). #P = 0.06, *P < 0.05, **P < 0.01, ***P < 0.001 versus control.