Activation of multiple signaling pathways causes developmental defects in mice with a Noonan syndrome–associated Sos1 mutation

Noonan syndrome (NS) is an autosomal dominant genetic disorder characterized by short stature, unique facial features, and congenital heart disease. About 10%–15% of individuals with NS have mutations in son of sevenless 1 (SOS1), which encodes a RAS and RAC guanine nucleotide exchange factor (GEF). To understand the role of SOS1 in the pathogenesis of NS, we generated mice with the NS-associated Sos1E846K gain-of-function mutation. Both heterozygous and homozygous mutant mice showed many NS-associated phenotypes, including growth delay, distinctive facial dysmorphia, hematologic abnormalities, and cardiac defects. We found that the Ras/MAPK pathway as well as Rac and Stat3 were activated in the mutant hearts. These data provide in vivo molecular and cellular evidence that Sos1 is a GEF for Rac under physiological conditions and suggest that Rac and Stat3 activation might contribute to NS phenotypes. Furthermore, prenatal administration of a MEK inhibitor ameliorated the embryonic lethality, cardiac defects, and NS features of the homozygous mutant mice, demonstrating that this signaling pathway might represent a promising therapeutic target for NS.

Noonan syndrome (NS), estimated to affect 1 out of 1,000–2,500 newborns, is characterized by variable developmental delay, short stature, characteristic facial features, congenital heart disease (CHD), and predisposition to myeloproliferative disease (MPD) (1–4). CHD is the primary cause of morbidity and mortality in NS patients. Pulmonary valve stenosis (PS) is the most common NS-associated cardiac phenotype, although some NS patients have cardiac hypertrophy and/or septal defects.

NS can occur sporadically or be inherited in an autosomal dominant fashion. Positional cloning and candidate gene approaches have identified NS-associated gene mutations in protein tyrosine phosphatase, non-receptor type 11 (PTPN11), son of sevenless 1 (SOS1), and RAF1 in about 60%–70% of NS cases, along with more rare mutations in N-RAS, K-RAS, and SHOC2 (5–12). Although additional NS genes remain to be identified, all the NS genes identified thus far encode mutant versions of RAS/MAPK pathway components that result in increased signaling in the pathway. Several related syndromes, also caused by mutations in RAS/MAPK family genes, share phenotypes with NS but also have significant differences (1). Although there is some purported genetic overlap, in most cases, mutations in different genes are associated with distinct syndromes. These disorders, now collectively referred to as the “Rasopathies,” show that germline mutations in members of the RAS/MAPK pathway lead to a phenotypically related set of genetic disorders (1, 13). A detailed understanding of the molecular etiology of these syndromes should yield new insights into the complexity of RAS/MAPK pathway regulation and is critical to intelligent pharmacological intervention.

About 10%–15% of NS cases are caused by gain-of-function mutations in SOS1 (7, 8), which encodes a bifunctional guanine nucleotide exchange factor (GEF) for RAS and RAC (14–18). A common NS-associated SOS1 missense mutation, E846K, is thought to cause a conformational change in the protein that leads to constitutive RASGEF activation (7, 8). Cultured cells transfected with the SOS1E846K mutant show sustained RAS activation and increased levels of phosphorylated ERK1/2 (p-ERK1/2) in response to EGF stimulation, suggesting that the RAS/MAPK pathway is activated by this mutation (8). Although most of the phenotypes in NS patients with the PTPN11 or SOS1 mutation are indistinguishable, PS and atrial septal defects (ASDs) are more prevalent in PTPN11 cases than in SOS1 cases, whereas those with SOS1-associated NS are less likely to have short stature or significant developmental delays (7, 8), suggesting that the underlying molecular effects of SOS1 and PTPN11 mutations might be overlapping but distinct.

As the RAS/MAPK pathway is also frequently affected by mutations in cancers, many inhibitors of pathway components are under development as antineoplastic agents (19–21). These inhibitors might be useful for treating the Rasopathies. Accurate mouse models of these disorders can be useful for prioritizing potential therapeutic agents.

To better understand the molecular pathogenesis of NS caused by SOS1 mutations and to enable evaluation of drug interventions to treat the phenotypic features of NS, we generated and characterized a mouse line carrying the Sos1E846K-activating mutation.

Generation of E846K mice by unidirectional recombination. The MAPK pathway is critical for many cellular functions throughout embryogenesis. To eliminate the possibility that expression of an activating Sos1 mutation might lead to dominant embryonic lethality, we introduced a silent Sos1E846K mutation into mouse ES cells that could be activated only when desired. To accomplish this goal, we used a unidirectional loxP recombination system in which selective recombination is achieved by means of a mutant loxP (mloxP) site that preferentially recombines with another mloxP but not with WT loxP (wloxP) and vice versa (22) (Figure 1A).

Figure 1

Generation of knock-in mice expressing Sos1^EK. (A) Schematic representation of unidirectional targeting of Sos1^EK in exon 16. When Cre recombinase is present, inversions occur either between the wloxP sites or the mloxP sites at opposite orientations, generating loxP sites with identical orientation. Subsequently, Cre recombinase mediates the excision between wloxP or mloxP sites in the same orientation, resulting in a targeted exon bearing the E846K mutation (solid triangles, wloxP; open triangles, mloxP; ovals, FRT site). Exons are indicated by rectangles. Primer pairs indicated by red, blue, green, and purple arrows were used in long-range PCR to screen positive ES cell clones (see Methods and Supplemental Figure 6). (B) Results of PCR of 2 ES cell clones with (and 1 clone without) Ad-Cre infection, showing that the desired modification has been achieved, using primer pairs indicated by orange arrows in A. M, DNA marker. Numbers indicate the size of PCR bands in kilo-base pairs (kb). (C) Sos1^EK activates Erk1/2 in 2 ES cell clones after Ad-Cre infection. ES cells were starved and stimulated with EGF (25 ng/ml) and analyzed by immunoblotting. (D) PCR results for genotyping the Sos1^EK-targeted locus, using primer pairs indicated by brown arrows in A. (E) Sequencing results of the RT-PCR of WT and Sos1^+/EK RNAs. The asterisk indicates the E846K mutation. (F) Immunoblotting showing equal amount of Sos1 proteins in tissues of all genotypes (1, WT; 2, Sos1^+/EK; 3, Sos1^EK/EK). (G) Kaplan-Meier survival curves of the mice. WT, n = 92; Sos1^+/EK, n = 137; P = 0.02 versus WT. Sos1^EK/EK, n = 4; P < 0.001 versus WT.

To test the efficacy of this strategy, we infected properly targeted ES cells with Cre recombinase–encoding adenovirus (Ad-Cre). Examination of genomic DNA from resulting colonies using long-range PCR revealed the desired inversion and recombination events (Figure 1B). Importantly, the level of Sos1 protein in the infected colonies was similar to that in the uninfected cells (Figure 1C). Moreover, p-Erk1/2 levels, an indicator of Erk activity, were increased in the infected cells in response to EGF stimulation (Figure 1C). These results indicated that the unidirectional recombination successfully introduced the functional E846K mutation into the ES cells.

We injected 2 ES clones (without Ad-Cre infection) into C57BL/6J blastocysts and obtained high-percentage chimeric mice that transmitted the modified allele to the germline. These mice were mated with EIIA-Cre transgenic mice, which express Cre recombinase in their germline (23). Examination of DNA from the resulting mice using PCR and sequencing revealed that the recombination events occurred as expected (Figure 1D and data not shown). RT-PCR with mRNA-specific primers, followed by sequencing as well as immunoblotting experiments, showed that the Sos1E846K allele was expressed at normal levels (Figure 1, E and F). We refer to the mutant locus as Sos1^EK.

Homozygosity of Sos1^EK results in severe cardiac defects and embryonic lethality. We obtained Sos1^+/EK mice at the expected Mendelian ratios from backcrosses with WT mice in a C57BL/6J background and from intercrosses of Sos1^+/EK mice in a mixed C57BL/6J and 129S1/SvImJ background (Table 1). However, the number of Sos1^EK/EK progeny from the intercross was significantly lower than the expected frequency (n = 3 out of 126 mice, 2.38%; χ² = 35.14, P < 0.0001; Table 1). Analysis of embryos at different stages of embryogenesis revealed that Sos1^EK/EK mice died at various stages from E10.5 to the neonatal stage (Table 1). Sos1^EK/EK embryos showed subcutaneous edema and hemorrhage, suggesting severe impairment of cardiovascular function (Figure 2A). Histological analysis of E15.5 Sos1^EK/EK embryos showed several cardiac defects, including secundum ASD (n = 5/5 embryos), ventricular septal defect (n = 4/5 embryos), aortic valvular (AV) stenosis (AS) due to thickened leaflets (n = 3/5 embryos), interventricular septal (IVS) and right ventricular hypertrophy (n = 3/5 embryos), and pericardial effusions (n = 5/5 embryos) (Figure 2B). Secundum ASD, a typical type of ASD diagnosed in NS patients, was also observed in Sos1^+/EK embryos, while only foramen ovale was observed in WT embryos (data not shown).

Figure 2

Cardiac defects in Sos1^EK/EK embryos. (A) Gross appearance of E13.5 embryos. (B) Representative H&E-stained transverse sections of hearts from E15.5 embryos. Note edema (asterisks), AS (red arrowhead), ASD (cyan arrows), ventricular septal defect (VSD, blue arrowhead), right ventricular hypertrophy (open circle), and IVS hypertrophy (open triangle) in Sos1^EK/EK hearts. PV, pulmonary valve. PV and AV original magnification, ×10. Four-Chamber sections original magnification, ×4.

Table 1

Progeny from the indicated Sos1^+/EK matings

To assess what part of the developing heart might be involved in leading to these phenotypes, we examined the expression patterns of Sos1 in this organ at E13.5. We observed that Sos1 protein was predominantly expressed in the aortic arch, pulmonary trunk, and the semilunar and the atrioventricular valves (Supplemental Figure 1; supplemental material available online with this article; doi: 10.1172/JCI43910DS1). This expression pattern was similar in the WT and mutant embryos (Supplemental Figure 1).

Developmental remodeling of cardiac tissues involves substantial cell proliferation and apoptosis at specific times and regions, such as in the developing valves of aortic and pulmonary trunk and the upper part of the IVS (24). We investigated cell proliferation using BrdU staining and apoptosis using TUNEL assay in the upper IVS region (Figure 3A) and AV leaflets (Figure 3B) in E15.5 embryos. At both sites, we observed increased BrdU labeling and a decreased number of TUNEL-positive cells in Sos1^EK/EK embryos compared with those of WT littermate controls, which could account for or contribute to the thickened AV and hypertrophic IVS (Figure 2B and Figure 3, A and B). These data suggest that aberrant patterns of cell proliferation and apoptosis are the cause of thickening of the semilunar valve and septal defects in the Sos1^EK/EK mice.

Figure 3

Cardiac defects in Sos1^EK/EK mice. (A and B) Immunofluorescence staining for BrdU (top rows) and TUNEL (bottom rows) in the (A) interventricular septum and (B) aortic valve leaflets of E15.5 embryos. Graphs show quantification with error bars indicating SD. *P < 0.05, **P < 0.01, ***P < 0.001 when compared with WT littermates. (C) H&E-stained sections showing AS in 3-month-old Sos1^EK/EK mice. (A–C) Original magnification, ×4. (D) Masson trichrome staining of heart tissue, showing fibrosis in 3-month-old Sos1^EK/EK mice. Note blue collagen staining in the left atrial (LA) epicardium (blue arrow) (left panel original magnification, ×4), with a magnified view in the middle panel (original magnification, ×20), as well as in right ventricular epicardium (right panel, original magnification, ×10). (E) Masson trichrome staining of Sos1^EK/EK hearts. Black arrows indicate degenerating cardiomyocytes. Blue arrows indicate adipocytes in the area surrounding (or around) the blood vessels (left panel, original magnification, ×10) and within the interventricular septum (right panel, original magnification, ×20).

The few Sos1^EK/EK mice that were born had a significantly lower life expectancy (Figure 1G). Histological analysis of these mice at the age of 3 months showed severe AS due to thickened leaflets (n = 3 mice; Figure 3C). Masson trichrome staining revealed epicardial and myocardial fibrosis (Figure 3D and Supplemental Figure 2) and degenerated myocardium (Figure 3E) in Sos1^EK/EK hearts. Interestingly, we observed a significant amount of adipocyte infiltration in the ventricular myocardium and in the surrounding blood vessels in the Sos1^EK/EK hearts (Figure 3E). None of these phenotypes were observed in WT littermates, whereas a small number of adipocytes were seen in the Sos1^+/EK hearts (data not shown). Taken together, these data suggest that Sos1^EK homozygosity caused multiple cardiac defects, similar to those in humans with NS, as well as a significant degree of embryonic lethality.

Sos1^+/EK mice develop cardiac hypertrophy. Although subcutaneous edema, hemorrhage, and pericardial effusion were observed in some Sos1^+/EK embryos (Figure 2, A and B), we obtained Sos1^+/EK mice at the expected frequency (Table 1). Histological analysis of Sos1^+/EK mice showed that some of them developed AS due to thickened leaflets, starting at the age of 8.5 months (n = 4/10 mice; Figure 4A). Although NS patients with SOS1 mutation develop PS, we did not observe PS in the Sos1^+/EK hearts (data not shown).

Figure 4

Cardiac defects in Sos1^+/EK mice. (A) Representative H&E-stained transverse sections, showing AS (arrow) in Sos1^+/EK hearts. Ao, aorta. Original magnification, ×10. (B) Representative Doppler image, with measurement of ascending aortic peak velocity of an 8-month-old Sos1^+/EK mouse. (C) Representative M-mode (left parasternal short axis view; top) and 2D (left parasternal long and short axes; bottom) echocardiographic images obtained from 8-month-old mice. Green bars (bottom) indicate the thickness of interventricular septum. IVS, IVS thickness; LVId, left ventricular dimension at diastole; LVIs, left ventricular dimension at systole; LVPW, left ventricular posterior wall thickness. (D–F) Representative Masson trichrome staining showing fibrosis in the hearts of a 9-month-old Sos1^+/EK mouse (right) but not in a WT littermate (left). (D) Original magnification, ×4. (E and F) Original magnification, ×10. (G) Immunofluorescence staining of collagen III (Col III) in the left ventricular wall. Original magnification, ×40. (H) TUNEL assay costained with the cardiomyocyte marker Troponin T, showing cardiomyocyte death in a Sos1^+/EK heart; the right panel shows a higher magnification view. Arrows indicate TUNEL positive cells. Original magnification, ×40 and ×64. (I) Representative quantitative real-time PCR results, showing increased expression of cardiac hypertrophic markers in the Sos1^+/EK hearts. Error bars indicate SD. **P < 0.01; ***P < 0.001.

With or without valve dysfunction, about 20% of SOS1 mutant-associated NS patients develop HCM (7, 8). We monitored male Sos1^+/EK mice using echocardiography monthly, beginning at 5 months. The echocardiograms of approximately 5- to 7-month-old WT and Sos1^+/EK mice were comparable (data not shown). At 8 months, while Doppler examinations of aortic blood velocity suggested normal valve function (130 ± 34.6 cm/s; Figure 4B), maximal wall thickness in the IVS was significantly increased in Sos1^+/EK mice (n = 7 mice) when compared with that of WT littermate controls (n = 5 mice) (1.0 ± 0.11 mm vs. 0.7 ± 0.07 mm; P < 0.001; Figure 4C and Table 2). In humans, the left ventricular end-diastolic diameter (LVDd) is indexed for the body weight to enable comparison among individuals with significantly different body size (25). Because of the significant differences in body weight between WT and Sos1^+/EK mice (see below), we normalized the LVDd to the body surface area (BSA) of the mice. The indexed parameters showed a significantly increased LVDd in Sos1^+/EK mice when compared with that of WT littermates. Taken together, these results indicated that Sos1^+/EK mice developed left ventricular hypertrophy (LVH; Table 2). Importantly, Sos1^+/EK mice had normal blood pressure, suggesting that hypertension was not the cause of their LVH (Supplemental Table 1).

Table 2

Echocardiographic parameters of 8-month-old mice

Wheat germ agglutinin staining of the Sos1^+/EK hearts at 8.5 months of age showed increased size and irregular shape of cardiomyocytes (Supplemental Figure 3). Masson trichrome staining of Sos1^+/EK hearts showed severe fibrosis of the left ventricular walls (Figure 4D), with both focal (Figure 4E) and interstitial (Figure 4F) fibrosis. Collagen III staining further confirmed significantly increased collagen deposits in Sos1^+/EK hearts (Figure 4G). In addition, the cardiomyocytes around the fibrotic areas showed TUNEL-positive nuclei, indicating cardiomyocyte death in the Sos1^+/EK mice (Figure 4H). Several hypertrophic markers, including atrial natriuretic factor (Anf), B-type natriuretic peptide (Bnp), and myosin heavy polypeptide 7, cardiac muscle, β (Myh7), were overexpressed in the Sos1^+/EK hearts (Figure 4I). Taken together, our results show that Sos1^+/EK mice develop pathological cardiac hypertrophy, which is also seen in NS patients.

Short stature and facial dysmorphia in Sos1^+/EK mice. NS is characterized by short stature (7, 8). Similarly, Sos1^+/EK and Sos1^EK/EK mutant mice showed gene dosage-dependent reduced body size, weight, and length, with preservation of overall body proportions when compared with WT littermates (Figure 5, A and B). As with NS patients, Sos1^+/EK and Sos1^EK/EK faces tended to be more “triangular” (Figure 5C), and mutant mice also tended to have blunter snouts caused by depression of the anterior frontal bone (Figure 5C). We further characterized the bone structure and quantified the actual dimensions of the mouse skull using CT scans (Figure 5D). Sos1^+/EK and Sos1^EK/EK mice had significantly shorter skull lengths and increased inner canthal distances when compared with those of WT littermates at the age of 3 months (Figure 5E).

Figure 5

Short stature and facial dysmorphia in Sos1^EK mice. (A) Representative appearance of 3-month-old littermates, showing reduced body length and size in Sos1 mutant mice. (B) Body weights of WT and mutant male mice with lines fit by linear regression. (P < 0.001 for Sos1^+/EK versus WT and P < 0.05 for Sos1^EK/EK versus WT.) (C) Facial defects in 3-month-old Sos1 mice. Arrowheads indicate blunt noses of mutant mice. (D) CT scans of 3-month-old mice. A sagittal projection (top row) and a coronal projection (bottom row) are shown. Numbers refer to the measurements shown in E. (E) Morphometric characteristics of the mice. Measurements were obtained on the CT sections at the same axial, coronal, and sagittal reference position in all mice (n = 3 for each genotype). Numbers are shown with SD. *P < 0.05, **P < 0.01, ***P < 0.001.

Hematological disorders in Sos1^+/EK mice. Individuals with NS often have unexplained hepatosplenomegaly and some develop MPD (3). By the age of 5 months, Sos1^+/EK mice developed splenomegaly (n = 8/8 mice; Figure 6A) resulting from extramedullary hematopoiesis, with increased erythropoiesis and increased numbers of megakaryocytes (Figure 6B). Blood smears from the Sos1^+/EK mice showed an increased number of neutrophils (n = 3/8 mice; Figure 6C). Sos1^+/EK mice showed a subtle but significant increase of leukocytes in peripheral blood counts when compared with WT littermates (4.74 ± 3.37 × 10³/μl vs. 2.06 ± 1.08 × 10³/μl; P < 0.05; Supplemental Table 2) at the age of 5 months. Flow cytometric analysis confirmed the myeloid expansion in the bone marrow and spleen of Sos1^+/EK mice (Figure 6D and Supplemental Figure 4). Bone marrow progenitors from Sos1^+/EK mice showed increased sensitivity to IL-3 or GM-CSF and also gave rise to factor-independent colonies (Figure 6E). These results indicate that Sos1^EK is associated with the development of hematological disorders.

Figure 6

Sos1^+/EK mice develop hematological disorder. (A) Representative view showing splenomegaly in Sos1^+/EK mice, with quantification graphed (n = 8). Error bars indicates SD. **P < 0.01. (B) Representative H&E-stained sections of spleens, showing increased numbers of megakaryocytes (white arrowheads) and massive increase of red pulp (bottom panel) in the Sos1^+/EK mice. Immunohistochemistry staining showing CD41-positive megakaryocytes (brown cells; right panels). Original magnification, ×75. (C) Blood smears showing an increased number of neutrophils in Sos1^+/EK mice. Original magnification, ×100. (D) Flow cytometric analysis of bone marrow and spleen, showing increased percentages of myeloid lineage (Mac1⁺ and Mac1⁺Gr1⁺) cells in Sos1^+/EK mice (P < 0.05 for bone marrow; P < 0.01 for spleen). Numbers indicate the percentage of positively-stained cells. (E) Increased myeloid colony formation of Sos1^+/EK bone marrow in the absence or presence of the indicated concentrations of GM-CSF or IL-3 (P < 0.05 and P < 0.01, respectively).

Signaling abnormalities in Sos1^EK hearts. Human cells that overexpress SOS1E846K show increased RasGEF activity, as manifested by sustained RAS activation and increased levels of p-ERK in response to EGF stimulation (8). Consistent with these findings, we observed increased Ras-GTP (Figure 7A) and p-Erk1/2 (Figure 7B) levels in Sos1^+/EK and Sos1^EK/EK hearts.

Figure 7

Altered signaling in Sos1^EK hearts. (A) Ras-GTP levels are increased in mutant hearts. (B) p-Erk1/2 levels are increased in mutant hearts. (C) Rac-GTP levels are increased in mutant hearts. (D) The phospho-kinase array detects phosphorylated proteins in WT and Sos1^+/EK heart lysates (left panel). The right panel shows higher magnification of numbered spots on the left panel. Note increased levels of p-Erk1/2, p-Mek, and p-Stat3 in Sos1^+/EK hearts. (E) Increased levels of p-Stat3 in the mutant hearts. (F) Immunofluorescence staining of p-Erk1/2 and p-Stat3 in heart sections from 3-month-old mice. Note colocalization of p-Erk1/2 and p-Stat3 with the DAPI stain. Original magnification, ×40. (G) STAT3 reporter assay showing increased STAT3 transcriptional activities in cells cotransfected with SOS1 or SOS1E846K. **P < 0.01, ***P < 0.01. neg, cotransfected with negative control vector. (H) Quantitative real-time PCR results showing increased expression of IL-6 in the Sos1^EK hearts. Error bars indicate SD. n = 3 for each genotype. **P < 0.01, ***P < 0.001 when compared with WT littermates.

Several studies have shown that Sos1 also can function as a RacGEF in transfected cell systems (14). We also observed a gene dosage-dependent increase in the level of endogenous Rac1-GTP in Sos1^+/EK and Sos1^EK/EK hearts (Figure 7C), providing the first evidence to our knowledge that Sos1^EK also functions as a RacGEF under physiologically relevant conditions.

We also asked whether Sos1^EK leads to increased activation of other MAPK pathway components or alterations in other signaling pathways that are associated with cardiac hypertrophy. We profiled kinase phosphorylation using phospho-kinase array (Figure 7D) and validated the results using immunoblotting. Interestingly, we found that the level of phosphorylated-Stat3 at Tyr705 (p-Stat3) was significantly increased in Sos1^+/EK and Sos1^EK/EK hearts (Figure 7, D and E). In contrast, we did not detect consistent activation of phosphorylation of Stat3 at Ser727, Stat5, p38, JNK, or Akt in all of the Sos1^EK hearts (Supplemental Figure 5). Both p-Erk1/2 and p-Stat3 can translocate to the nucleus and increase transcriptional initiation (26, 27). We observed increased nuclear staining of both p-Erk1/2 and p-Stat3 in Sos1^+/EK and Sos1^EK/EK hearts (Figure 7F). In addition, overexpression of SOS1E846K increased the transcriptional activities of STAT3 when compared with WT SOS1 in human cells (Figure 7G), which further confirmed the activation of STAT3 by SOS1E846K mutation.

Previous studies suggest that Rac1 can induce STAT3 phosphorylation at Tyr705, but not at Ser727, by promoting autocrine production of IL-6 (28, 29). Interestingly, we detected increased expression of IL-6 in Sos1^+/EK and Sos1^EK/EK hearts (Figure 7H). These results suggest that Sos1^EK increased Rac activity that might induce Stat3 activation through autocrine IL-6. Erk1/2 and Stat3 are known to be involved in cardiac hypertrophy (30–33), and it is likely that both signaling molecules contribute to the cardiac phenotypes observed in the Sos1^EK mice.

Prenatal treatment with MEK inhibitor improves the embryonic lethality and NS features in Sos1^EK/EK mice. We asked whether treating pregnant mothers with a small-molecule RAS/MAPK pathway inhibitor could diminish the embryonic lethality or phenotypic features of Sos1 mutant offspring. PD0325901 selectively binds to and inhibits MEK, resulting in reduction of the phosphorylation and activation of Erk1/2 (19, 34–36). PD0325901 was shown to be efficient in preventing tumor incidence and growth in mice at a dosage of 1 mg/kg of body weight (mg/kg) (37). Therefore, we administered PD0325901 (1 mg/kg) daily i.p. to pregnant mice from Sos1^+/EK intercrosses (n = 6 mice), beginning at 7 days post coitum and continuing (in nursing females) until P9. Beginning at P10, the drug was injected individually into pups (i.p.) until P28. Prenatal administration of the drug completely rescued the prenatal lethality of homozygous mice, such that they were born at the expected Mendelian ratio (n = 9 out of 36 mice, 25%; χ² = 2.67, P = 0.263; Table 1). We examined 3 Sos1^EK/EK mice at P2 and found a normal heart structure in 2 of the mice, while the third had died and had a secundum ASD (Figure 8A). Immunoblotting indicated that the phosphorylation of Erk1/2 had been normalized, whereas p-Stat3 levels remained high in Sos1^EK/EK hearts (Figure 8B). One Sos1^EK/EK mouse died at P14 with a secundum ASD and hypertrophic right ventricles with dilated epicardial vessels (Figure 8C). The rest of the Sos1^EK/EK mice survived (n = 5 mice) and, unlike untreated Sos1^EK/EK mice, had no anatomical abnormalities in their hearts at the age of 3 months (n = 3 mice; Figure 8D).

Figure 8

Prenatal treatment with the MEK inhibitor blocks development of NS features. (A) H&E-stained sections of hearts from PD0325901-treated mice at P2. The asterisk indicates ASD. Top panel, original magnification, ×20. Bottom panel, original magnification, ×4. (B) Immunoblot showing effects of PD0325901 treatment on Erk1/2 and Stat3 activation. (C) H&E-stained section of a PD0325901-treated Sos1^EK/EK mouse that died at P14. Note ASD (arrow), hypertrophic right ventricle wall (asterisk), and dilated epicardial vessels (arrowhead). (D) Representative sections of heart tissue of 3-month-old Sos1^EK/EK mice with or without PD0325901 treatment. H&E-stained sections showing AS in the untreated mice (black arrowhead; row 1, original magnification, ×10). Masson Trichrome–stained sections showing fibrosis in untreated mice (rows 2–4). Note blue collagen staining (blue arrow) in left ventricular epicardium (row 2, original magnification, ×4), right atrial (RA) epicardium (row 3, original magnification, ×10), and right ventricular epicardium and myocardium (row 4, original magnification, ×10). Scale bar: 500 μm. (E) Body weight of treated and untreated male mice fit by linear regression. P < 0.01 for both PD0325901-treated Sos1^+/EK mice versus PD0325901-treated WT mice and PD0325901-treated Sos1^EK/EK mice versus PD0325901-treated WT mice. PD, PD0325901. (F) CT scans of treated and control mice (top row). A sagittal projection (top row) and a coronal projection (bottom row) are shown. Skull measurements are shown as in Figure 5E. *P < 0.05 when compared with WT.

The body weight of PD0325901-treated Sos1^EK/EK mice increased and was comparable to either treated or untreated Sos1^+/EK mice but was still lower when compared with either treated or untreated WT mice (Figure 8E). Furthermore, CT scans of the skull of the treated Sos1^+/EK and Sos1^EK/EK mice showed improvement of their facial dysmorphia at the age of 3 months (Figure 8F).

These data suggest that prenatal inhibition of Erk1/2 activation by the MEK inhibitor PD0325901 successfully rescued the embryonic lethality and reduced the penetrance of cardiac defects of Sos1^EK/EK mice.

We describe here what we believe to be the first mouse model for NS caused by Sos1 mutation. Analogous to that in humans, heterozygosity of this mutation leads to a number of phenotypes, such as reduced growth, facial dysmorphia, hematological defects, and cardiac hypertrophy. We observed that in the heart, Sos1^EK leads to Ras activation and, importantly, Rac activation as well. In addition to increased activation of the Ras/MAPK pathway, we unexpectedly observed increased activation of Stat3. Homozygous Sos1^EK mutation resulted in a severe NS phenotype, with some embryos dying as early as E10.5, whereas others were born and lived for at least 3 months. Examination of late-stage Sos1^EK/EK embryos revealed a spectrum of severe cardiac abnormalities.

Distinct phenotypes of Sos1^EK mice. Our studies indicate that the phenotypes in Sos1^EK mice and previous NS-associated Ptpn11^D61G or other Ptpn11 mouse models were overlapping to some degree but distinguishable (38–43). First, Ptpn11^D61G/D61G were completely embryonic lethal, whereas we obtained a small number of Sos1^EK/EK mice (41). Second, we observed several distinct cardiac phenotypes in Sos1^EK/EK mice, such as epicardial fibrosis and adipocyte infiltration, which were not detected in any of the Ptpn11 models (38–43). Third, LVH was not documented in Ptpn11 models; indeed, mice with homozygous Ptpn11^D61G or heterozygous Ptpn11^D61Y mutations have dilated cardiomyopathy (41, 42). By contrast, Sos1^+/EK mice developed LVH and ventricular fibrosis. Although the majority of the Sos1^+/EK mice with AS showed LVH, we also observed LVH in several Sos1^+/EK mice without AS and with normal blood pressure.

SOS1^EK mutations appeared to have selective effect on semilunar valves, whereas Ptpn11^DG mice had more of an effect on atrioventricular valves. In addition, although PS is the major clinical cardiac feature of NS patients carrying SOS1 mutation, neither histological analysis nor Doppler echocardiography detected PS in our mouse model (data not shown). On the other hand, AS was observed frequently in Sos1^EK/EK mice and in older Sos1^+/EK mice. Finally, in addition to activation of Ras and Erk, we found that Rac and Stat3 were also activated in Sos1^EK mice, which was not found in the Ptpn11 models (38–40, 44). It is possible, if not likely, that the common phenotypes observed in Sos1 and Ptpn11 models, such as growth delay, craniofacial features, hematological disorders, and some cardiac defects, were due to the enhanced Erk activation seen in both models, whereas the distinct features in the Sos1 model described above were due to the selective activation of Rac and/or Stat3.

Distinct molecular signaling in Sos1^EK mice. SOS1 not only has a RasGEF activity but can act as a RacGEF in cultured cells. These GEF activities are mediated by distinct Cdc25 homology and Dbl homology (DH) domains, respectively (45, 46), in SOS1. Rac is a key molecule in integrating diverse stimuli and transducing signals in the cardiovascular system (47). Several studies indicate that Rac activation can contribute to cardiac hypertrophy in vitro and in vivo (48–52). Our study provides the first in vivo evidence to our knowledge for Rac activation by gain-of-function Sos1 proteins under endogenous levels of expression and suggests a role for Rac activation in the pathogenesis of Sos1-associated NS. Since the E846 residue is not in the Rac-binding DH domain of the Sos1 protein, the precise mechanism of the activation of Rac needs further investigation.

Our study also suggests that Stat3 activation contributes to the cardiac defects in our mouse model. The transcription factor Stat3 participates in a wide range of physiological processes, including proliferation, apoptosis, tumor growth, and angiogenesis (26). Stat3 activation also promotes cardiomyocyte survival and hypertrophy (31). Stat3 can be activated by the G protein–coupled receptor via Rac1 (53–60). Some studies suggested that Rac1 directly binds to and regulates STAT3 (61, 62), while other studies suggested that Rac1 induces cytokine secretion that activates STAT3 (28, 29, 63). Our data suggest that Sos1^EK mutation causes Rac activation and increased production of IL-6, leading to Stat3 activation in the heart.

Small-molecule treatments for Sos1^EK mice. Several studies suggest that targeting RAS/MAPK signaling might be a powerful therapeutic strategy against cardiac hypertrophy (64). Our data provide what we believe to be the first example of successful rescue of embryonic lethality and reduced penetrance of cardiac defects by prenatal treatment with a small-molecule MEK inhibitor in a NS mouse model. However, some cardiac defects still exist in the Sos1^EK/EK mice, possibly because of the persistently activated Stat3. Inhibitors for Rac and/or Stat3 may be necessary to further relieve the cardiac defects in Sos1^EK/EK mice. Previously, the use of the MEK inhibitor U0126 in a transgenic Ptpn11 NS mouse model during pregnancy reduced the craniofacial defects (40). Our PD0325901-treated Sos1^EK mice also showed improved skull shape. The growth of the PD0325901-treated Sos1^EK/EK mice was improved but not normalized. In fact, the growth of the PD0325901-treated Sos1^+/EK mice remained the same as that of the untreated ones, suggesting that inhibiting Erk1/2 during pregnancy and early age might not be sufficient for normalizing the short statue in NS. Our previous studies showed that strong mutant alleles of PTPN11 cause both perinatal and postnatal growth defects, whereas others caused only postnatal defects (41, 42). Together with the present Sos1 model, our studies suggest that there is a distinct pathogenesis of these growth defects.

In summary, our data suggested that Ras and Erk1/2, as well as Rac and Stat3, are activated and may all contribute to the cardiac defects of the Sos1^EK mouse models. Normalizing all of these pathways might be required for successful amelioration of the entire spectrum of observed phenotypes. Given the gene-specific differences in NS phenotypes that our mouse models have revealed (41, 42), it also is possible that distinct treatment regimes will be warranted for different NS patients based on the specific genetic mutation they carry.

View Supplemental data

We thank Libin Wang, Elizabeth Sparks, Katarina Riesner, Shuyan Wang, and Erica Tworog-Dube for technical assistance. This work is supported by NIH grant CA 84301 (to R. Kucherlapati), NHLBI NIH Syscode and LeDucq Foundation (to J.G. Seidman), NIH grants CA49132 and HL083273 (to B.G. Neel), and HHMI and LeDucq Foundation (to C.E. Seidman).

Address correspondence to: Raju Kucherlapati, Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Suite 160, Boston, Massachusetts 02115, USA. Phone: 617.525.4445; Fax: 617.525.4440; E-mail: rkucherlapati@partners.org.

Conflict of interest: The authors have declared that no conflict of interest exists.

Reference information: J Clin Invest. 2010;120(12):4353–4365. doi:10.1172/JCI43910.

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