Nucleotide pool imbalance and adenosine deaminase deficiency induce alterations of N-region insertions during V(D)J recombination
Lisa Gangi-Peterson, … , Thomas B. Kepler, Beverly S. Mitchell
Lisa Gangi-Peterson, … , Thomas B. Kepler, Beverly S. Mitchell
Published March 15, 1999
Citation Information: J Clin Invest. 1999;103(6):833-841. https://doi.org/10.1172/JCI4320.
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Article

Nucleotide pool imbalance and adenosine deaminase deficiency induce alterations of N-region insertions during V(D)J recombination

Abstract

Template-independent nucleotide additions (N regions) generated at sites of V(D)J recombination by terminal deoxynucleotidyl transferase (TdT) increase the diversity of antigen receptors. Two inborn errors of purine metabolism, deficiencies of adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP), result in defective lymphoid development and aberrant pools of 2′-deoxynucleotides that are substrates for TdT in lymphoid precursors. We have asked whether selective increases in dATP or dGTP pools result in altered N regions in an extrachromosomal substrate transfected into T-cell or pre–B-cell lines. Exposure of the transfected cells to 2′-deoxyadenosine and an ADA inhibitor increased the dATP pool and resulted in a marked increase in A–T insertions at recombination junctions, with an overall decreased frequency of V(D)J recombination. Sequence analysis of VH-DH-JH junctions from the IgM locus in B-cell lines from ADA-deficient patients demonstrated an increase in A–T insertions equivalent to that found in the transfected cells. In contrast, elevation of dGTP pools, as would occur in PNP deficiency, did not alter the already rich G–C content of N regions. We conclude that the frequency of V(D)J recombination and the composition of N-insertions are influenced by increases in dATP levels, potentially leading to alterations in antigen receptors and aberrant lymphoid development. Alterations in N-region insertions may contribute to the B-cell dysfunction associated with ADA deficiency.

Authors

Lisa Gangi-Peterson, David H. Sorscher, Jon W. Reynolds, Thomas B. Kepler, Beverly S. Mitchell

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Analysis of V(D)J recombinants from Jurkat cells treated with 2′-deoxyad...
Analysis of V(D)J recombinants from Jurkat cells treated with 2′-deoxyadenosine. T cells transfected with either pSJ-F1 or pCJ-F1 were exposed to 3 μM dCF and 50 μM dAdo for 18 h. Sequences of N-region insertions from 10 illustrative recombinants are presented for both signal and coding joints. The G–C content of N-region insertions from controls and nucleoside-treated recombinants was determined by dividing the total number of G + C nucleotides by the total number of A + T nucleotides in each N region. Comparison of the G + C/A + T ratio from controls with the G + C/A + T ratio from dAdo-treated recombinants was significant at P < 10–4 for pSJ-F1 and P = 0.04 for pCJ-F1. P nucleotides were present in 12% of the coding junctions. The omission of P nucleotides did not significantly alter the probability calculation. Subtraction of P nucleotides resulted in the following G + C/A + T ratios at N-region coding joints: control = 1.9; dAdo-treated = 1.02. A 2 × 2 contingency table analysis of G–C composition of 27 independent recombinants containing N-region insertions from signal joints and 30 independent recombinants containing N-region insertions from coding joints is presented. The P value determined for equivalent N-region addition to each strand versus no effect, according to the Fisher's exact test, was P = 0.0004 (signal joint) and P = 1.0 (coding joint).