Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells
Robert E. Hunger, … , Steven A. Porcelli, Robert L. Modlin
Robert E. Hunger, … , Steven A. Porcelli, Robert L. Modlin
Published March 1, 2004
Citation Information: J Clin Invest. 2004;113(5):701-708. https://doi.org/10.1172/JCI19655.
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Article Dermatology

Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells

Abstract

Langerhans cells (LCs) constitute a subset of DCs that initiate immune responses in skin. Using leprosy as a model, we investigated whether expression of CD1a and langerin, an LC-specific C-type lectin, imparts a specific functional role to LCs. LC-like DCs and freshly isolated epidermal LCs presented nonpeptide antigens of Mycobacterium leprae to T cell clones derived from a leprosy patient in a CD1a-restricted and langerin-dependent manner. LC-like DCs were more efficient at CD1a-restricted antigen presentation than monocyte-derived DCs. LCs in leprosy lesions coexpress CD1a and langerin, placing LCs in position to efficiently present a subset of antigens to T cells as part of the host response to human infectious disease.

Authors

Robert E. Hunger, Peter A. Sieling, Maria Teresa Ochoa, Makoto Sugaya, Anne E. Burdick, Thomas H. Rea, Patrick J. Brennan, John T. Belisle, Andrew Blauvelt, Steven A. Porcelli, Robert L. Modlin

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Comparison of antigen presentation by LC-like DCs and MD-DCs. LC-like DC...
Comparison of antigen presentation by LC-like DCs and MD-DCs. LC-like DCs (filled symbols) or MD-DCs (open symbols) were incubated with T cells in the presence of the indicated amount of M. leprae or M. tuberculosis sonicates. (A) Proliferation was measured as described for Figure 2. (B) IFN-γ production was measured as described in Methods. The values shown are the mean ± SEM of triplicate cultures and are representative of at least three independent experiments.