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Article has an altmetric score of 3

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Referenced in 11 patents
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Research Article Free access | 10.1172/JCI188

Mobilization and homing of peripheral blood progenitors is related to reversible downregulation of alpha4 beta1 integrin expression and function.

F Prosper, D Stroncek, J B McCarthy, and C M Verfaillie

Stem Cell Biology Program and Division of Hematology, Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Find articles by Prosper, F. in: JCI | PubMed | Google Scholar

Stem Cell Biology Program and Division of Hematology, Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Find articles by Stroncek, D. in: JCI | PubMed | Google Scholar

Stem Cell Biology Program and Division of Hematology, Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Find articles by McCarthy, J. in: JCI | PubMed | Google Scholar

Stem Cell Biology Program and Division of Hematology, Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Find articles by Verfaillie, C. in: JCI | PubMed | Google Scholar

Published June 1, 1998 - More info

Published in Volume 101, Issue 11 on June 1, 1998
J Clin Invest. 1998;101(11):2456–2467. https://doi.org/10.1172/JCI188.
© 1998 The American Society for Clinical Investigation
Published June 1, 1998 - Version history
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Abstract

Despite the wide use of mobilized peripheral blood (PB) progenitor cells (PBPC) for clinical transplantation the mechanism(s) underlying their mobilization and subsequent engraftment are still unknown. We compared the adhesive phenotype of CD34(+) colony-forming cells (CFC) in bone marrow (BM) and PB of normal donors before and after administration of granulocyte colony-stimulating factor (G-CSF) for 5 d. G-CSF-mobilized PB CFC cells adhered significantly less to BM stroma, fibronectin, and to the alpha4 beta1 binding fibronectin peptide, CS1, because of decreased expression of the alpha4 integrin. Since incubation of BM CD34(+) cells for 4 d with G-CSF at concentrations found in serum of G-CSF- treated individuals did not affect alpha4-dependent adhesion, G-CSF may not be directly responsible for the decreased alpha4-mediated adhesion of PB CFC. Culture of G-CSF-mobilized PB CD34(+) cells with cytokines at concentrations found in BM stromal cultures upregulated alpha4 expression and restored adhesion of mobilized PB CFC to stroma, fibronectin, and CS1. Adhesion of cultured, mobilized PB CFC to stroma and CS1 could not be further upregulated by the beta1 activating antibody, 8A2. This indicates acquisition of a maximally activated alpha4 beta1 integrin once PB CFC have been removed from the in vivo mobilizing milieu. Thus, decreased alpha4 expression on CD34(+) CFC in PB may be responsible for the aberrant circulation of mobilized PB CD34(+) cells. Reexpression of a maximally activated alpha4 beta1 integrin on mobilized PB CFC removed from the mobilizing in vivo milieu may contribute to the early engraftment of mobilized PBPC.

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Referenced in 11 patents
30 readers on Mendeley
See more details