(A) scRNA sequencing data of liver cells from 5 healthy donors — 3 AC and 5 sAH — and peripheral WBC from 3 donors with AC and 4 donors with sAH. In total, 87,105 cells were analyzed, integrated, and clustered by Seurat. UMAP plots of each group are shown. (B) Neutrophils (CD16⁺CD114⁺, Cluster 0 in Figure 1A) from all groups were reclustered. UMAP plots of all the neutrophils from each group are shown. (C) Feature plots for the gene expression of CXCL8 (a gene encoding IL-8 protein) among all groups of neutrophils. (D) sAH and AC liver tissues were stained with IL-8 and neutrophil marker MPO. Representative images are shown. The number and percentage of IL-8⁺ neutrophils are presented as means ± SEM. ****P < 0.0001. Scale bars: 50 μm. (E) Heatmap of genes that related to IL-8 upregulation in neutrophils from scRNA-Seq data. (F) sAH liver tissues were stained with IL-8, phospho-p38 MAPK, and neutrophil marker MPO. A representative merged image is shown, and the image with staining with each individual antibody is shown in Supplemental Figure 3D. Scale bars: 20 μm. (G) Heatmap of neutrophil chemokine and cell maker genes from scRNA-Seq data.