Flow cytometric detection of C5aR and FcγRII/III on AMs. (a) Control cells from peripheral blood (PBC) and resident AMs isolated from BAL fluid of C57BL/6 WT and C5aR–/– mice were stained with the newly developed anti-C5aR mAb 20/70 conjugated to FITC in combination with PE anti-FcγRII/III 2.4G2 mAb and analyzed on a FACScan. (b) MH-S AM cells were cultured under 10% FCS medium conditions. Simultaneous expression of FcγRII/III and C5aR was detected by FACS analysis using PE-2.4G2 and FITC-20/70 mAbs.