(A) T_AN cells are enriched in islet allografts and spleens of tolerized recipients. n = 6–12 per group. (B) Loss of T_AN cells in islet allografts following MCMV infection of stably tolerized recipients. n = 3–9 per group. (C) T_AN cells from tolerized recipients are induced to produce IFN-γ by MCMV-infected DCs (representative of 2 independent experiments). (D) In vivo conversion of adoptively transferred CD45.2 T_AN cells following MCMV infection of CD45.1 hosts. n = 2–3 per group. (E) Depletion of T_AN cells prevents MCMV-mediated transplant tolerance disruption. n = 3–5 per group. (F) Experimental scheme in RAG^–/– mice. (G) In vivo conversion of adoptively transferred T_AN cells following MCMV infection of RAG^–/– (representative of n = 4). (H) Rejection of islet allografts in RAG^–/– following T_AN adoptive transfer. n = 3–4 per group. *P < 0.05. (A–D) Mann-Whitney test. (E) Log-rank test.