Northern blot analysis of ABCG5 and ABCG8 in male and female transgenic mice. Total RNA was isolated from the liver and jejunum of mice on a chow or 2% cholesterol diet. Equal amounts of total RNA from each mouse in each group were pooled and subjected to Northern blotting as described for Figure 1b. This experiment was repeated, and the results were similar. WT, wild-type transgene.