IL-12 enhances the natural killer cell cytokine response to Ab-coated tumor cells
Robin Parihar, … , Yan Hu, William E. Carson
Robin Parihar, … , Yan Hu, William E. Carson
Published October 1, 2002
Citation Information: J Clin Invest. 2002;110(7):983-992. https://doi.org/10.1172/JCI15950.
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IL-12 enhances the natural killer cell cytokine response to Ab-coated tumor cells

Abstract

Research Article

Authors

Robin Parihar, Julie Dierksheide, Yan Hu, William E. Carson

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IL-12 is the most effective costimulus with immobilized IgG for IFN-γ pr...
IL-12 is the most effective costimulus with immobilized IgG for IFN-γ production by NK cells. Purified NK cells were cultured on immobilized huIgG in the presence of various human cytokines (IL-2, IL-10, IL-15, IL-18, and IL-12) at 10 ng/ml. Standard controls were also included. As a further control, some wells were coated with human F(ab′)2 fragment prior to the addition of NK cells (Fab). Culture supernatants were harvested after 72 hours and analyzed for IFN-γ content by ELISA. *P < 0.005 versus all conditions shown.