Hiroshi Yamamoto, Charlotte E. Lee, Jacob N. Marcus, Todd D. Williams, J. Michael Overton, Marisol E. Lopez, Anthony N. Hollenberg, Laurie Baggio, Clifford B. Saper, Daniel J. Drucker, Joel K. Elmquist
EXN-4–activated neurons projecting to the spinal cord. A series of line drawings demonstrates the distribution of Fos-IR (black circles), FG-IR (blue circles), and double-labeled (red stars) neurons in three rostral-to-caudal levels of the PVH (a–c), the Arc/RCA (d–f), and the LHA (g–i) of the hypothalamus after i.c.v. injection of EXN-4, in animals with FG injection into the spinal cord. Scale bar = 250 μm in a–c and 1,000 μm in d–i. Divisions of the PVH: dp, dorsal parvocellular; mp, medial parvocellular; pm, posterior magnocellular; vp, ventral parvocellular; lp, lateral parvicellular; ic, internal capsule. VMH, ventromedial nucleus of the hypothalamus; DMH, dorsomedial nucleus of the hypothalamus; ME, median eminence.