Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Rapid nontranscriptional activation of endothelial nitric oxide synthase mediates increased cerebral blood flow and stroke protection by corticosteroids
Florian P. Limbourg, … , Michael A. Moskowitz, James K. Liao
Florian P. Limbourg, … , Michael A. Moskowitz, James K. Liao
Published December 1, 2002
Citation Information: J Clin Invest. 2002;110(11):1729-1738. https://doi.org/10.1172/JCI15481.
View: Text | PDF | Erratum
Article Vascular biology

Rapid nontranscriptional activation of endothelial nitric oxide synthase mediates increased cerebral blood flow and stroke protection by corticosteroids

  • Text
  • PDF
Abstract

Research Article

Authors

Florian P. Limbourg, Zhihong Huang, Jean-Christophe Plumier, Tommaso Simoncini, Masayuki Fujioka, Jan Tuckermann, Günther Schütz, Michael A. Moskowitz, James K. Liao

×

Figure 1

Options: View larger image (or click on image) Download as PowerPoint
Acute neuroprotection by corticosteroids requires eNOS. (a) Dose–depende...
Acute neuroprotection by corticosteroids requires eNOS. (a) Dose–dependent effects of dexamethasone (Dex) on stroke volume derived from TTC-stained (24 hours) or hematoxylin and eosin–stained (72 hours) brain sections after MCAo (n = 8–11). *P < 0.05 vs. vehicle. (b) Effects of delayed steroid treatment on neuroprotection. Shown is a schema of treatment regimens and corresponding cerebral infarct reduction by dexamethasone relative to the vehicle (Veh) group (n = 7–11). *P < 0.05 vs. vehicle. (c) Infarct volume corrected for edema in wild-type and eNOS–/– mice treated with vehicle or dexamethasone (20 mg/kg bolus administered intraperitoneally [i.p.]) 24 hours after MCAo (n = 5–11). Wild-type mice are identical to those in (a). *P < 0.05 vs. all other groups; WT, wild-type. (d) The eNOS protein and mRNA levels after steroid treatment. Equal amounts of aortic tissue homogenates (40 μg) were immunoblotted against eNOS followed by stripping and reprobing against actin (top panels). A representative experiment is shown. Three separate experiments yielded similar results. Brain eNOS mRNA expression from mice treated with vehicle or dexamethasone with or without RU486 (RU) or LY294002 (LY) for 24 hours is shown in the lower panel. The corrected eNOS mRNA levels (eNOS/GAPDH) were determined by real time PCR (n ≥ 3 for each condition). t, time.

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts