The c-kit expression in vitro and in SVZ and SGZ of normal and ischemic rat brain in vivo. Normoxic cerebral cortical cultures (a) and cultures deprived of oxygen for 8 hours (b) were stained with Ab’s against c-kit and SCF and with DAPI. Brain sections through SVZ (c–f) and SGZ of DG (g–j) were also immunostained with an Ab against c-kit, which was visualized with DAB. c-kit was expressed in SVZ (c and e) and SGZ (g and i) of normal brain (Con), and expression was increased 24 hours after MCA occlusion (Isch) in the ipsilateral SVZ (d and f) and SGZ (h and j). (c, d, g, and h) ×200. (e, f, i, and j) ×400 (HP). Data are representative fields from at least three experiments per panel.