KO of the CT/CGRP gene results in increased bone in mice. (a) Radiographic and histologic analysis of vertebrae from 1-month-old and 3-month-old WT and KO mice, and of the proximal tibiae from 3-month-old WT and KO mice. (b) Histomorphometric analysis of trabecular bone volume in vertebrae of WT (gray bars) and KO (black bars) mice. Graphs provide data for trabecular bone volume as a ratio of total bone volume (BV/TV), trabecular number (TbN), trabecular thickness (TbTh), and trabecular spacing (TbSp). Bars represent mean ± SD (n = 5). *Statistically significant difference between WT and KO animals (P < 0.05) as determined by Student t test.