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  • Persistent brain injury in sepsis survivors
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Commentary Free access | 10.1172/JCI127679

The vasculature in sepsis: delivering poison or remedy to the brain?

Benjamin H. Singer

Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA.

Address correspondence to: Benjamin H. Singer, Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, 4067 Biomedical Science Research Building, 109 Zina Pitcher Place, Ann Arbor, Michigan 48109, USA. Phone: 734.764.4554; Email: singerb@med.umich.edu.

Find articles by Singer, B. in: JCI | PubMed | Google Scholar |

Published March 18, 2019 - More info

Published in Volume 129, Issue 4 on April 1, 2019
J Clin Invest. 2019;129(4):1527–1529. https://doi.org/10.1172/JCI127679.
© 2019 American Society for Clinical Investigation
Published March 18, 2019 - Version history
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Related article:

Circulating heparan sulfate fragments mediate septic cognitive dysfunction
Joseph A. Hippensteel, … , Paco S. Herson, Eric P. Schmidt
Joseph A. Hippensteel, … , Paco S. Herson, Eric P. Schmidt
Concise Communication Neuroscience Article has an altmetric score of 21

Circulating heparan sulfate fragments mediate septic cognitive dysfunction

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Abstract

Septic patients frequently develop cognitive impairment that persists beyond hospital discharge. The impact of sepsis on electrophysiological and molecular determinants of learning is underexplored. We observed that mice that survived sepsis or endotoxemia experienced loss of hippocampal long-term potentiation (LTP), a brain-derived neurotrophic factor–mediated (BDNF-mediated) process responsible for spatial memory formation. Memory impairment occurred despite preserved hippocampal BDNF content and could be reversed by stimulation of BDNF signaling, suggesting the presence of a local BDNF inhibitor. Sepsis is associated with degradation of the endothelial glycocalyx, releasing heparan sulfate fragments (of sufficient size and sulfation to bind BDNF) into the circulation. Heparan sulfate fragments penetrated the hippocampal blood-brain barrier during sepsis and inhibited BDNF-mediated LTP. Glycoarray approaches demonstrated that the avidity of heparan sulfate for BDNF increased with sulfation at the 2-O position of iduronic acid and the N position of glucosamine. Circulating heparan sulfate in endotoxemic mice and septic humans was enriched in 2-O– and N-sulfated disaccharides; furthermore, the presence of these sulfation patterns in the plasma of septic patients at intensive care unit (ICU) admission predicted persistent cognitive impairment 14 days after ICU discharge or at hospital discharge. Our findings indicate that circulating 2-O– and N-sulfated heparan sulfate fragments contribute to septic cognitive impairment.

Authors

Joseph A. Hippensteel, Brian J. Anderson, James E. Orfila, Sarah A. McMurtry, Robert M. Dietz, Guowei Su, Joshay A. Ford, Kaori Oshima, Yimu Yang, Fuming Zhang, Xiaorui Han, Yanlei Yu, Jian Liu, Robert J. Linhardt, Nuala J. Meyer, Paco S. Herson, Eric P. Schmidt

×

Abstract

Survivors of sepsis and other forms of critical illness frequently experience significant and disabling cognitive and affective disorders. Inflammation, ischemia, and glial cell dysfunction contribute to this persistent brain injury. In this issue of the JCI, Hippensteel et al. show that endothelial injury in animal models of sepsis or endotoxemia leads to shedding of heparan fragments from the endothelial glycocalyx. These fragments directly sequester brain-derived neurotrophic factor and impair hippocampal long-term potentiation, an electrophysiologic correlate of memory. The authors further explore the specific characteristics of heparan fragments that bind neurotrophins and the presence of these fragments in the circulation of patients who survive sepsis. This study highlights an important mechanism by which vascular injury can impair brain function.

Persistent brain injury in sepsis survivors

Mortality from sepsis has fallen markedly with improvements in supportive care (1). Although over 14 million patients survive a hospitalization for sepsis each year, they are still not made whole by the life-saving care they receive (2). Rather, sepsis survivors frequently experience functional disability, along with increased risk of rehospitalization and death. Persistent brain dysfunction is a common and life-changing consequence of critical illness survivorship and results in both cognitive decline and a high incidence of affective disorders such as anxiety, depression, and posttraumatic stress (3, 4).

Multiple factors contribute to persistent brain dysfunction in sepsis survivors. Neuropathologic studies of sepsis in patients have largely been confined to those who succumb to their acute illness and have revealed that ischemia, hemorrhage, and white matter injury commonly occur at the extremes of critical illness (5). It is unclear, however, whether brain injury that results from the hypoperfusion, hypoxia, and circulatory dysfunction that underlie multiorgan dysfunction syndrome in sepsis is treatable or preventable by means other than improved supportive care of the underlying critical illness. Studies using animal models, therefore, have focused heavily on inflammatory mechanisms that extend beyond the period of critical illness and into the period of survivorship. Neuroinflammation, including activation of microglia and astrocytes, is also a prominent component of brain injury in sepsis (6, 7).

Survivors of endotoxemia, abdominal infection, or pneumonia experience multiple persistent inflammatory insults to the brain. Neutrophils and inflammatory monocytes traffic and invade the brain parenchyma (8). Expression of cytokines and alarmins in the brain persists for weeks following sepsis (9, 10). Microglia are especially susceptible to long-term reprogramming after high-dose endotoxin exposure (11), though the balance of tolerance and priming that results from persistent exposure to proinflammatory signals over time is probably governed by a network of interactions among glia and neurons (12). In addition to the activation of intrinsic neuroinflammatory responses, sepsis may result in increased blood-brain barrier permeability and entry of inflammatory signals from the circulation (13, 14).

Despite evidence pointing to multiple mechanisms of persistent brain injury in sepsis survivors, the connection from a panoply of vascular and inflammatory insults to an actual persistent, reversible neuronal dysfunction in sepsis survivors remains obscure. Cytokine signaling and direct activation of innate immune receptors play an important role in the maintenance and elimination of synapses in both normal physiology and disease (15, 16), and these same mechanisms are presumably at work in the brains of sepsis survivors. Inhibition of IL-1 signaling and microglial activation rescues impairment of the long-term potentiation (LTP) that occurs acutely after sepsis or endotoxemia (17, 18). Though cytokines act directly on neurons and glia to change their intrinsic physiology and structure, inflammation may also activate a cascade of potentially reversible dysfunctions in the processing of neurotrophins and other signals important for circuit homeostasis. For example, brain-derived neurotrophic factor (BDNF) is an agonist of the TrkB receptor kinase that is required for long-lasting facilitation of synaptic transmission in LTP. In aged animals, systemic infection has been associated with prolonged increases in IL-1β expression and reductions in hippocampal BDNF, as well as sustained but reversible impairment of LTP (19).

BDNF sequestration as a mechanism of learning and memory impairment

In this issue of the JCI, Hippensteel and colleagues demonstrate that microvascular injury in sepsis results in impairment of learning and memory by a far more direct mechanism: sequestration of BDNF by products of endothelial glycocalyx injury (20). The endothelial glycocalyx is a meshwork of extracellular protein and carbohydrate molecules that supports endothelial function (21). Sepsis degrades the glycocalyx and leads to pathologic vascular permeability and leukocyte adhesion. Here, however, the authors found that heparan sulfate fragments, constituents of the glycocalyx itself, interfered with brain function. Circulating heparan fragments were elevated in patients with sepsis and were associated with cognitive impairment in sepsis survivors. Murine sepsis survivors demonstrated deficits in hippocampus-dependent behavior and LTP impairment. While total levels of hippocampal BDNF were not reduced after sepsis, sulfated heparan fragments functionally sequester BDNF (Figure 1). The authors were not able to specifically disrupt the interaction of heparan sulfate fragments and BDNF to restore LTP. However, supplementation of BDNF ex vivo reversed the electrophysiologic effect of endotoxemia, and direct TrkB agonism overcame the inhibitory effect of highly sulfated heparans on LTP.

Injury to the endothelial glycocalyx in sepsis results in the release of heFigure 1

Injury to the endothelial glycocalyx in sepsis results in the release of heparan sulfate fragments into the brain parenchyma. Heparan sulfate fragments then bind and sequester BDNF, preventing activation of the TrkB receptor and leading to the loss of downstream TrkB signaling and protein synthesis that support synaptic strengthening during LTP. Illustrated by Rachel Davidowitz.

Endothelial injury products in future studies of brain injury

The possibility that products of endothelial injury can interfere directly with brain function has several implications for future studies dealing with the prevention and treatment of brain injury associated with sepsis. The dominant model of persistent brain injury in sepsis ascribes neuronal dysfunction to a combination of circulatory, immune, and glial dysregulation. Cognitive dysfunction has been associated with vascular injury during sepsis, but this has usually been framed in terms of hemodynamic impairment or loss of barrier function (14, 22). The complex relationships among peripherally derived inflammatory mediators and leukocytes, microglia, and astrocytes in sepsis survivors that impinge on neuronal function depend on cascades of signaling pathways and changes in transcriptional programs that alter intrinsic neuronal function and expression of neurotrophins and physically alter synaptic structure. Therefore, therapies should be directed either at preventing brain injury — by minimizing ischemia or improving cerebrovascular endothelial function — or at modulating the complex neuroimmune response that persists in sepsis survivors.

The findings of Hippensteel and colleagues do not refute the importance of ischemia and neuroinflammation, but do highlight a far more direct route by which endothelial injury can interfere in the function of neuronal networks. The authors made the striking observation that, since heparan sulfate fragments circulate, endothelial injury need not originate in the cerebral microcirculation to have a significant effect on brain function. Other groups have also recently found that products of endothelial injury, such as pulmonary endothelial amyloids produced in critically ill patients with pneumonia, enter the CSF and can impair LTP (23).

Search for other components released in tissue injury

If these neurotoxic products can originate anywhere in the body during critical illness that results in vascular injury, then what type of therapeutic strategies are needed to protect or restore function? While this study focuses on BDNF, we can speculate that glycocalyx fragments and extracellular matrix components released in tissue injury may bind to and inhibit other signals that are important for synaptic function. Preventing glycocalyx shedding entirely, given that patients with sepsis are already experiencing endothelial injury when they present for care, is not a practical strategy. Alternatively, a fuller understanding of which factors are bound by glycocalyx fragments would identify targets for pharmacologic interventions that might speed recovery from brain dysfunction in sepsis survivors. Finally, direct disruption of the physical sequestration of neurotrophins by heparan sulfate fragments would not only augment neurotrophin signaling, but also restore it in the very synapses where it is needed. Though the authors were not able to effectively reverse heparan sulfate fragment binding to BDNF in their study, their results suggest that this could be a powerful new direction for therapeutic research.

Persistent brain dysfunction in sepsis survivors is reflected in cognitive and affective disorders that cause significant disability, reduce quality of life, and contribute to morbidity and mortality. The mainstay of treating acute brain dysfunction has for many years been to alter neuromodulatory neurotransmission, mainly with typical or atypical antipsychotics. Recent landmark studies have demonstrated that this approach fails to improve brain function or alter short-term mortality (24, 25). While data on long-term outcomes are still forthcoming, the inability of antipsychotic medications to reverse or prevent delirium illustrates that simply masking behavior, such as agitation, will not restore the important homeostatic mechanisms that the brain needs to recover from acute injuries and prevent long-term dysfunction. We need a more nuanced understanding of the underlying pathophysiology of brain injury in survivors of critical illness, and here Hippensteel and colleagues have cast new light on previously unexplored but important mechanisms.

Acknowledgments

BHS is supported by the NIH (K08NS101054) and the American Thoracic Society Foundation.

Address correspondence to: Benjamin H. Singer, Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, 4067 Biomedical Science Research Building, 109 Zina Pitcher Place, Ann Arbor, Michigan 48109, USA. Phone: 734.764.4554; Email: singerb@med.umich.edu.

Footnotes

Conflict of interest: The author has declared that no conflict of interest exists.

Copyright: © 2019 American Society for Clinical Investigation

Reference information: J Clin Invest. 2019;129(4):1527–1529. https://doi.org/10.1172/JCI127679.

See the related article at Circulating heparan sulfate fragments mediate septic cognitive dysfunction.

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Version history
  • Version 1 (March 18, 2019): Electronic publication
  • Version 2 (April 1, 2019): Print issue publication

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  • Top
  • Abstract
  • Persistent brain injury in sepsis survivors
  • BDNF sequestration as a mechanism of learning and memory impairment
  • Endothelial injury products in future studies of brain injury
  • Search for other components released in tissue injury
  • Acknowledgments
  • Footnotes
  • References
  • Version history
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