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Research Article Free access | 10.1172/JCI118481

Differentiated vascular myocytes: are they involved in neointimal formation?

B Holifield, T Helgason, S Jemelka, A Taylor, S Navran, J Allen, and C Seidel

Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.

Find articles by Holifield, B. in: JCI | PubMed | Google Scholar

Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.

Find articles by Helgason, T. in: JCI | PubMed | Google Scholar

Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.

Find articles by Jemelka, S. in: JCI | PubMed | Google Scholar

Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.

Find articles by Taylor, A. in: JCI | PubMed | Google Scholar

Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.

Find articles by Navran, S. in: JCI | PubMed | Google Scholar

Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.

Find articles by Allen, J. in: JCI | PubMed | Google Scholar

Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.

Find articles by Seidel, C. in: JCI | PubMed | Google Scholar

Published February 1, 1996 - More info

Published in Volume 97, Issue 3 on February 1, 1996
J Clin Invest. 1996;97(3):814–825. https://doi.org/10.1172/JCI118481.
© 1996 The American Society for Clinical Investigation
Published February 1, 1996 - Version history
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Abstract

The role of differentiated vascular myocytes are neointimal formation in canine carotid artery was investigated. Using antibodies and cDNA probes, cells were characterized in situ and after isolation. In situ characterization indicated the majority of medial cells expressed both smooth muscle myosin and alpha actin but many cells were negative to these markers. All adventitial cells were negative for these proteins. The muscle protein-positive cells were designated differentiated, vascular myocytes (VSMC). The others were designated type 2 cells. Sequential enzyme digestion from lumenal surface yielded VSMC ( > 90%) while digestions from the adventitial surface yielded type 2 cells ( > 90%). VSMC were viable in culture but did not spread, proliferate, or alter expression of muscle proteins. Type 2 cells proliferated and increased their expression of muscle actin but did not express muscle myosin. Characterization of neointimal cells from injured carotid arteries indicated they were morphologically and immunologically identical to cultured type 2 cells. We concluded that: (a) canine carotid artery media consists of a heterogeneous cell population: (b) serum does not stimulate isolated VSMC to undergo phenotypic modulation or proliferate: and (c) type 2 cells may be responsible for neointimal formation because they proliferate and acquire a phenotype identical to in situ neointimal cells.

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