Advertisement

Research Article Free access | 10.1172/JCI118476

Chronic treatment with interleukin-1 beta induces coronary intimal lesions and vasospastic responses in pigs in vivo. The role of platelet-derived growth factor.

H Shimokawa, A Ito, Y Fukumoto, T Kadokami, R Nakaike, M Sakata, T Takayanagi, K Egashira, and A Takeshita

Research Institute of Angiocardiology, Kyushu University School of Medicine, Fukuoka, Japan.

Find articles by Shimokawa, H. in: JCI | PubMed | Google Scholar

Research Institute of Angiocardiology, Kyushu University School of Medicine, Fukuoka, Japan.

Find articles by Ito, A. in: JCI | PubMed | Google Scholar

Research Institute of Angiocardiology, Kyushu University School of Medicine, Fukuoka, Japan.

Find articles by Fukumoto, Y. in: JCI | PubMed | Google Scholar

Research Institute of Angiocardiology, Kyushu University School of Medicine, Fukuoka, Japan.

Find articles by Kadokami, T. in: JCI | PubMed | Google Scholar

Research Institute of Angiocardiology, Kyushu University School of Medicine, Fukuoka, Japan.

Find articles by Nakaike, R. in: JCI | PubMed | Google Scholar

Research Institute of Angiocardiology, Kyushu University School of Medicine, Fukuoka, Japan.

Find articles by Sakata, M. in: JCI | PubMed | Google Scholar

Research Institute of Angiocardiology, Kyushu University School of Medicine, Fukuoka, Japan.

Find articles by Takayanagi, T. in: JCI | PubMed | Google Scholar

Research Institute of Angiocardiology, Kyushu University School of Medicine, Fukuoka, Japan.

Find articles by Egashira, K. in: JCI | PubMed | Google Scholar

Research Institute of Angiocardiology, Kyushu University School of Medicine, Fukuoka, Japan.

Find articles by Takeshita, A. in: JCI | PubMed | Google Scholar

Published February 1, 1996 - More info

Published in Volume 97, Issue 3 on February 1, 1996
J Clin Invest. 1996;97(3):769–776. https://doi.org/10.1172/JCI118476.
© 1996 The American Society for Clinical Investigation
Published February 1, 1996 - Version history
View PDF
Abstract

Studies in vitro have suggested that inflammatory cytokines may play an important role in the pathogenesis of atherosclerosis. However, little is known about their effects in vivo. Thus, the present study was designed to determine in vivo what histological and functional changes may be induced by chronic treatment with IL-1 beta, one of the major inflammatory cytokines, and also to clarify what mechanisms are involved in those changes. Under aseptic conditions, proximal segments of the left porcine coronary arteries were gently wrapped with cotton mesh absorbing Sepharose beads either with or without recombinant human IL-1 beta. From 1 to 4 wk after the operation, coronary vasospastic responses to intracoronary serotonin or histamine were noted at the IL-1 beta-treated site but not at the control site. Histologically, intimal thickening was greater at the IL-1 beta-treated site than at the control site. Those functional and histological changes induced by the chronic treatment with IL-1 beta were significantly inhibited by the simultaneous treatment with a neutralizing antibody to either IL-1 beta or PDGF. These results indicate that chronic treatment with Il-1 beta induces coronary intimal lesions and vasospastic responses in porcine coronary arteries in vivo and also suggest that these changes are substantially mediated by PDGF.

Version history
  • Version 1 (February 1, 1996): No description
Advertisement
Advertisement