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Article has an altmetric score of 3

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Referenced in 8 patents
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Research Article Free access | 10.1172/JCI118282

Two different cis-acting regulatory regions direct cell-specific transcription of the collagen alpha 1(I) gene in hepatic stellate cells and in skin and tendon fibroblasts.

K Houglum, M Buck, J Alcorn, S Contreras, P Bornstein, and M Chojkier

Department of Medicine, Veterans Affairs Medical Center, San Diego, California, USA.

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Department of Medicine, Veterans Affairs Medical Center, San Diego, California, USA.

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Department of Medicine, Veterans Affairs Medical Center, San Diego, California, USA.

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Department of Medicine, Veterans Affairs Medical Center, San Diego, California, USA.

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Department of Medicine, Veterans Affairs Medical Center, San Diego, California, USA.

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Department of Medicine, Veterans Affairs Medical Center, San Diego, California, USA.

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Published November 1, 1995 - More info

Published in Volume 96, Issue 5 on November 1, 1995
J Clin Invest. 1995;96(5):2269–2276. https://doi.org/10.1172/JCI118282.
© 1995 The American Society for Clinical Investigation
Published November 1, 1995 - Version history
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Abstract

The expression of the collagen alpha 1(I) gene in activated stellate cells plays an important role during liver fibrogenesis. To identify the critical cis-elements of the collagen alpha 1(I) gene in stellate cells, we used transgenic animals bearing various collagen alpha 1(I) regulatory regions directing the expression of either a human growth hormone minigene or the bacterial beta-galactosidase gene. We found that collagen alpha 1(I)-human growth hormone transgene expression was constitutively high in tendon and skin, provided the transgene contained the -2.3 to -0.44 kb collagen regulatory region. However in the liver, expression was stimulated several-fold, as was the endogeneous gene, by the fibrogenic hepatotoxin carbon tetrachloride. This stimulation occurred whether the collagen 5' regulatory region extended -2.3, -1.6 or -0.44 kb, and in the presence or absence of much of the first intron (+292 to +1607 bp). In addition, the -0.44 kb 5' region was sufficient for high-level transgene expression in stellate cells, following their activation by culture on plastic. In contrast, in skin and tendon, high-level transcription of the collagen alpha 1(I) gene required the -2.3 to -0.44 kb 5' flanking region. Thus, two different cis-regulatory regions direct cell-specific transcription of the collagen alpha 1(I) gene in stellate cells and in skin and tendon.

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Referenced in 8 patents
7 readers on Mendeley
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