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Research Article Free access | 10.1172/JCI118248

Impairment of selectin-mediated leukocyte adhesion to venular endothelium in spontaneously hypertensive rats.

M Suematsu, H Suzuki, T Tamatani, Y Iigou, F A DeLano, M Miyasaka, M J Forrest, R Kannagi, B W Zweifach, and Y Ishimura

Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.

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Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.

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Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.

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Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.

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Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.

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Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.

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Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.

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Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.

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Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.

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Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.

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Published October 1, 1995 - More info

Published in Volume 96, Issue 4 on October 1, 1995
J Clin Invest. 1995;96(4):2009–2016. https://doi.org/10.1172/JCI118248.
© 1995 The American Society for Clinical Investigation
Published October 1, 1995 - Version history
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Abstract

The present study was designed to elucidate whether molecular mechanisms for leukocyte adhesion to microvascular endothelium may differ between spontaneously hypertensive rats and Wistar Kyoto rats. Leukocyte rolling and adhesion were investigated while monitoring venular wall shear rates in the mesenteric microcirculation stimulated with histamine or tert-butyl hydroperoxide in the two strains. In Wistar Kyoto rats, 10 microM histamine as well as 500 microM tertbutyl hydroperoxide promoted a significant reduction of venular leukocyte rolling velocity and subsequent adhesion. These changes in leukocyte behavior were blocked by monoclonal antibodies against P-selectin (PB 1.3) and against sialyl Lewis X-like carbohydrates (2H5). However, spontaneously hypertensive rats exhibited a blunted response of the stimulus-elicited leukocyte rolling, which was associated with impairment of venular P-selectin expression as well as a decrease in the expression of sialyl Lewis X-like carbohydrates on circulating neutrophils. No significant differences were detected between the two strains not only in the surface CD11b/CD18 expression but also in the CD18-mediated adhesivity of neutrophils to intracellular adhesion molecule-1 transfectants in vitro. These results suggest that impairment of selectin-mediated leukocyte adhesion is an event responsible for disorders of inflammatory responses in spontaneously hypertensive rats.

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