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Referenced in 7 patents
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Research Article Free access | 10.1172/JCI118009

Acid aspiration-induced lung injury in rabbits is mediated by interleukin-8-dependent mechanisms.

H G Folkesson, M A Matthay, C A Hébert, and V C Broaddus

Cardiovascular Research Institute, University of California San Francisco 94143-0130, USA.

Find articles by Folkesson, H. in: JCI | PubMed | Google Scholar

Cardiovascular Research Institute, University of California San Francisco 94143-0130, USA.

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Cardiovascular Research Institute, University of California San Francisco 94143-0130, USA.

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Cardiovascular Research Institute, University of California San Francisco 94143-0130, USA.

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Published July 1, 1995 - More info

Published in Volume 96, Issue 1 on July 1, 1995
J Clin Invest. 1995;96(1):107–116. https://doi.org/10.1172/JCI118009.
© 1995 The American Society for Clinical Investigation
Published July 1, 1995 - Version history
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Abstract

Acid aspiration lung injury may be mediated primarily by neutrophils recruited to the lung by acid-induced cytokines. We hypothesized that a major acid-induced cytokine was IL-8 and that a neutralizing anti-rabbit-IL-8 monoclonal antibody (ARIL8.2) would attenuate acid-induced lung injury in rabbits. Hydrochloric acid (pH = 1.5 in 1/3 normal saline) or 1/3 normal saline (4 ml/kg) was instilled into the lungs of ventilated, anesthetized rabbits. The rabbits were studied for 6 or 24 h. In acid-instilled rabbits without the anti-IL-8 monoclonal antibody, severe lung injury developed in the first 6 h; in the long-term experiments, all rabbits died with lung injury between 12 and 14 h. In acid-instilled rabbits given the anti-IL-8 monoclonal antibody (2 mg/kg, intravenously) either as pretreatment (5 min before the acid) or as treatment (1 h after the acid), acid-induced abnormalities in oxygenation and extravascular lung water were prevented and extravascular protein accumulation was reduced by 70%; in the long-term experiments, anti-IL-8 treatment similarly protected lung function throughout the 24-h period. The anti-IL-8 monoclonal antibody also significantly reduced air space neutrophil counts and IL-8 concentrations. This study establishes IL-8 as a critical cytokine for the development of acid-induced lung injury. Neutralization of IL-8 may provide the first useful therapy for this clinically important form of acute lung injury.

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Referenced in 7 patents
90 readers on Mendeley
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