Regulation of rat mesangial cell growth by diadenosine phosphates.

S Heidenreich; M Tepel; H Schlüter; B Harrach; W Zidek

doi:10.1172/JCI117992

Med. Univ.-Poliklinik, Institut für Arterioskleroseforschung, University of Münster, Germany.

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Med. Univ.-Poliklinik, Institut für Arterioskleroseforschung, University of Münster, Germany.

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Med. Univ.-Poliklinik, Institut für Arterioskleroseforschung, University of Münster, Germany.

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Med. Univ.-Poliklinik, Institut für Arterioskleroseforschung, University of Münster, Germany.

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Med. Univ.-Poliklinik, Institut für Arterioskleroseforschung, University of Münster, Germany.

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Published in Volume 95, Issue 6 on June 1, 1995
J Clin Invest. 1995;95(6):2862–2867. https://doi.org/10.1172/JCI117992.
© 1995 The American Society for Clinical Investigation

Published June 1, 1995 - Version history

The newly recognized human endogenous vasoconstrictive dinucleotides, diadenosine pentaphosphate (AP5A) and diadenosine hexaphosphate (AP6A), were tested for growth stimulatory effects in rat mesangial cells (MC). Both AP5A and AP6A stimulated growth in micromolar concentrations. The growth stimulatory effect exceeded that of ATP, alpha,beta-methylene ATP, adenosine 5'-O-(3-thio)triphosphate and UTP. Both diadenosine phosphates potentiated the growth response to platelet-derived growth factor, but not to insulin-like growth factor-1. To further elucidate the site of action in the cell cycle, RNA and protein synthesis were assessed. AP5 and AP6A stimulated protein synthesis, but not RNA formation. Furthermore, both agents increased cytosolic free Ca2+ concentration. It is concluded that AP5A and AP6A may play a regulatory role in MC growth as progression factors and possibly modify MC proliferation in glomerular disease.