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Citations to this article

Glucose-induced alterations of cytosolic free calcium in cultured rat tail artery vascular smooth muscle cells.
M Barbagallo, … , P K Pang, L M Resnick
M Barbagallo, … , P K Pang, L M Resnick
Published February 1, 1995
Citation Information: J Clin Invest. 1995;95(2):763-767. https://doi.org/10.1172/JCI117724.
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Research Article

Glucose-induced alterations of cytosolic free calcium in cultured rat tail artery vascular smooth muscle cells.

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Abstract

We have previously suggested that hyperglycemia per se may contribute to diabetic hypertensive and vascular disease by altering cellular ion content. To more directly investigate the potential role of glucose in this process, we measured cytosolic free calcium in primary cultures of vascular smooth muscle cells isolated from Sprague-Dawley rat tail artery before and after incubation with 5 (basal), 10, 15, and 20 mM glucose. Glucose significantly elevated cytosolic free calcium in a dose- and time-dependent manner, from 110.0 +/- 5.4 to 124.5 +/- 9.0, 192.7 +/- 20.4, and 228.4 +/- 21.9 nM at 5, 10, 15, and 20 mM glucose concentrations, respectively. This glucose-induced cytosolic free calcium elevation was also specific, no change being observed after incubation with equivalent concentrations of L-glucose or mannitol. This glucose effect was also dependent on extracellular calcium and pH, since these calcium changes were inhibited in an acidotic or a calcium-free medium, or by the competitive calcium antagonist lanthanum. We conclude that ambient glucose concentrations within clinically observed limits may alter cellular calcium ion homeostasis in vascular smooth muscle cells. We suggest that these cellular ionic effects of hyperglycemia may underlie the predisposition to hypertension and vascular diseases among diabetic subjects and/or those with impaired glucose tolerance.

Authors

M Barbagallo, J Shan, P K Pang, L M Resnick

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Total citations by year

Year: 2020 2019 2017 2016 2015 2014 2013 2012 2010 2009 2008 2007 2006 2004 2003 2002 2001 1999 1998 1997 1996 1995 Total
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Citations to this article (55)

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