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Citations to this article

Enhanced interleukin-8 release and gene expression in macrophages after exposure to Mycobacterium tuberculosis and its components.
Y Zhang, … , J Reibman, W N Rom
Y Zhang, … , J Reibman, W N Rom
Published February 1, 1995
Citation Information: J Clin Invest. 1995;95(2):586-592. https://doi.org/10.1172/JCI117702.
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Enhanced interleukin-8 release and gene expression in macrophages after exposure to Mycobacterium tuberculosis and its components.

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Abstract

Mycobacterium tuberculosis infection is accompanied by acute and chronic inflammatory infiltrates associated with necrotizing granulomas in lung tissue. The cellular infiltrate is characterized by inflammatory cells which include neutrophils, lymphocytes, and macrophages. In animal and in vitro models of mycobacterial infection, cytokines including tumor necrosis factor-alpha (TNF-alpha), interferon gamma (IFN-gamma), and interleukin-1 beta (IL-1 beta) participate in granulomatous inflammation. We hypothesized that interleukin-3, a potent chemoattractant for neutrophils and lymphocytes, could be released by activated alveolar macrophages after exposure to M. tuberculosis or its components and contribute to granulomatous lung inflammation. A quantitative immunoassay revealed that IL-8 protein release was significantly elevated in supernatants of macrophages and in lavage fluid obtained from patients with pulmonary tuberculosis compared to normal controls. In addition, Northern blots demonstrated striking up-regulation of IL-8 mRNA in macrophages from these patients. M. tuberculosis and its cell wall components lipoarabinomannan (LAM), lipomannan (LM), and phosphoinositolmannoside (PIM) stimulated IL-8 protein release and mRNA expression in vitro from alveolar macrophages, but deacylated LAM did not. Neutralizing antibodies to TNF-alpha and/or IL-1-alpha and beta blocked 83% of the stimulation. IL-8 synthesis and release is an early response of macrophages after phagocytosis of M. tuberculosis. Its production serves to attract both acute and chronic inflammatory cells of active infection and thus participates in the process of containment of the pathogen.

Authors

Y Zhang, M Broser, H Cohen, M Bodkin, K Law, J Reibman, W N Rom

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Journal of immunology (Baltimore, Md. : 1950) 2003
Granulomatous Infections and Inflammations: Cellular and Molecular Mechanisms
DL Boros
2003
mRNA Expression of Cytokines and Chemokines in Herniated Lumbar Intervertebral Discs
SH Ahn, YW Cho, MW Ahn, SH Jang, YK Sohn, HS Kim
Spine 2002
Differential Regulation of Chemokine Secretion in Tuberculous and Staphylococcal Osteomyelitis
KM Wright, JS Friedland
Journal of Bone and Mineral Research 2002
Signals Involved in Mycobacteria-Induced CXCL-8 Production by Human Monocytes
P Méndez-Samperio, J Palma, A Vázquez
Journal of Interferon & Cytokine Research 2002
Innate immunity to Mycobacterium tuberculosis
R Crevel, TH Ottenhoff, JW van der Meer
Clinical microbiology reviews 2002
<scp>d</scp>-ARABINOFURANOSIDES FROM MYCOBACTERIA: SYNTHESIS AND CONFORMATION
TL Lowary
Journal of Carbohydrate Chemistry 2002
Elevated Levels of α-Defensins in Plasma and BAL Fluid of Patients With Active Pulmonary Tuberculosis
J Ashitani, H Mukae, T Hiratsuka, M Nakazato, K Kumamoto, S Matsukura
Chest 2002
Structural Study of Lipomannan and Lipoarabinomannan from Mycobacterium chelonae: PRESENCE OF UNUSUAL COMPONENTS WITH α1,3-MANNOPYRANOSE SIDE CHAINS
Y Guérardel, E Maes, E Elass, Y Leroy, P Timmerman, GS Besra, C Locht, G Strecker, L Kremer
The Journal of biological chemistry 2002
Glycomicrobiology
RJ Doyle
2002
Induction of TNF in Human Alveolar Macrophages As a Potential Evasion Mechanism of Virulent Mycobacterium tuberculosis
M Engele, E Stöβel, K Castiglione, N Schwerdtner, M Wagner, P Bölcskei, M Röllinghoff, S Stenger
Journal of immunology (Baltimore, Md. : 1950) 2002
Mycobacterium bovis BCG Vaccination Augments Interleukin-8 mRNA Expression and Protein Production in Guinea Pig Alveolar Macrophages Infected with Mycobacterium tuberculosis
MJ Lyons, T Yoshimura, DN McMurray
Infection and immunity 2002
Interleukin-8 Secretion from Mycobacterium tuberculosis -Infected Monocytes Is Regulated by Protein Tyrosine Kinases but Not by ERK1/2 or p38 Mitogen-Activated Protein Kinases
C Ameixa, JS Friedland
Infection and immunity 2002
Mycobacterium avium Complex Promotes Recruitment of Monocyte Hosts for HIV-1 and Bacteria
H Hale-Donze, T Greenwell-Wild, D Mizel, TM Doherty, D Chatterjee, JM Orenstein, SM Wahl
Journal of immunology (Baltimore, Md. : 1950) 2002
Regulation of Interleukin-8 by Interleukin-10 and Transforming Growth Factor β in Human Monocytes Infected with Mycobacterium bovis
P Méndez-Samperio, E García, A Vázquez, J Palma
Clinical and vaccine immunology : CVI 2002
Increased Mycobacterium tuberculosis growth in HIV-1-infected human macrophages: role of tumour necrosis factor-alpha
FG Imperiali, A Zaninoni, LL Maestra, P Tarsia, F Blasi, W Barcellini
Clinical & Experimental Immunology 2001
Priming of human neutrophils by mycobacterial lipoarabinomannans: role of granule mobilisation
J Fäldt, C Dahlgren, M Ridell, A Karlsson
Microbes and Infection 2001
Roles of Intracellular Calcium and NF-κB in the Bacillus Calmette- Guérin-Induced Secretion of Interleukin-8 from Human Monocytes
P Méndez-Samperio, J Palma, A Vázquez
Cellular Immunology 2001
Inflammation
JA Hunt
Encyclopedia of Materials Science and Technology 2001
Expression of IL-15 in inflammatory pulmonary diseases
S Muro, R Taha, A Tsicopoulos, R Olivenstein, AB Tonnel, P Christodoulopoulos, B Wallaert, Q Hamid
Journal of Allergy and Clinical Immunology 2001
Mononuclear cells ingest E. coli opsonized by investigational intravenous immunoglobulin preparations in the absence of complement more efficiently than polymorphonuclear phagocytes
C Tinguely, M Schaller, UE Nydegger
Transfusion and Apheresis Science 2001
Activation of Peripheral Blood Neutrophils from Patients with Active Advanced Tuberculosis
M Alemán, M Beigier-Bompadre, C Borghetti, S la Barrera, E Abbate, M Isturiz, MC Sasiain
Clinical Immunology 2001
Human Immunodeficiency Virus–Seronegative Adults with Extrapulmonary Tuberculosis Have Abnormal Innate Immune Responses
TR Sterling, SE Dorman, RE Chaisson, L Ding, J Hackman, K Moore, SM Holland
Clinical Infectious Diseases 2001
Plasma and BAL Fluid Concentrations of Antimicrobial Peptides in Patients With Mycobacterium avium- intracellulare Infection
J Ashitani, H Mukae, T Hiratsuka, M Nakazato, K Kumamoto, S Matsukura
Chest 2001
Glycoscience: Chemistry and Chemical Biology I–III
BO Fraser-Reid, K Tatsuta, J Thiem
2001
Down-Regulation of Interleukin-8 Secretion from Mycobacterium tuberculosis -Infected Monocytes by Interleukin-4 and -10 but Not by Interleukin-13
JM Mansfield, C Ameixa, JS Friedland
Infection and immunity 2001
Induction of Inducible Nitric Oxide Synthase-NO· by Lipoarabinomannan of Mycobacterium tuberculosis Is Mediated by MEK1-ERK, MKK7-JNK, and NF-κB Signaling Pathways
JT Barbieri, ED Chan, KR Morris, JT Belisle, P Hill, LK Remigio, PJ Brennan, DW Riches
Infection and immunity 2001
URINARY INTERLEUKIN-8 AND 18 PREDICT THE RESPONSE OF SUPERFICIAL BLADDER CANCER TO INTRAVESICAL THERAPY WITH BACILLUS CALMETTE-GUERIN
GN Thalmann, A Sermier, C Rentsch, K M??Hrle, MG Cecchini, UE Studer
The Journal of Urology 2000
Hut Lung
JA Gold, J Jagirdar, JG Hay, DJ Addrizzo-Harris, DP Naidich, WN Rom
Medicine 2000
Clinical investigation of pulmonary Mycobacterium avium complex infection in human T lymphotrophic virus type I carriers
W Matsuyama, A Mizoguchi, F Iwami, Y Koreeda, J Wakimoto, H Kanazawa, S Mori, M Kawabata, H Fukunaga, M Osame
Thorax 2000
Differential tolerance induction by lipoarabinomannan and lipopolysaccharide in human macrophages
DD Riedel, SH Kaufmann
Microbes and Infection 2000
Mycobacterial Lipoarabinomannan Induces an Inflammatory Response in the Mouse Lung: A Role for Interleukin-1
NP Juffermans, A Verbon, JT Belisle, PJ Hill, P Speelman, SA van DEVENTER, TO van der POLL
American journal of respiratory and critical care medicine 2000
LPS and LAM activation of the U373 astrocytoma cell line: Differential requirement for CD14
SL Orr, P Tobias
Journal of Endotoxin Research 2000
Mycobacterium tuberculosis H37Rv Parietal and Cellular Lipoarabinomannans: CHARACTERIZATION OF THE ACYL- AND GLYCO-FORMS
M Gilleron, L Bala, T Brando, A Vercellone, G Puzo
The Journal of biological chemistry 2000
Development of an Asthma Vaccine: Research into BCG
CB Scanga, GL Gros
Drugs 2000
CRL-1072 Enhances Antimycobacterial Activity of Human Macrophages Through Interleukin-8
C Jagannath, S Pai, JK Actor, RL Hunter
Journal of Interferon & Cytokine Research 1999
Influence of polymorphism in the genes for the interleukin (IL)-1 receptor antagonist and IL-1beta on tuberculosis
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Journal of Experimental Medicine 1999
Lipoarabinomannans: characterization of the multiacylated forms of the phosphatidyl-myo-inositol anchor by NMR spectroscopy
J Nigou, M Gilleron, G Puzo
Biochemical Journal 1999
Roles of lipoarabinomannan in the pathogenesis of tuberculosis
GR Strohmeier, MJ Fenton
Microbes and Infection 1999
Influence of Polymorphism in the Genes for the Interleukin (IL)-1 Receptor Antagonist and IL-1  on Tuberculosis
RJ Wilkinson, P Patel, M Llewelyn, CS Hirsch, G Pasvol, G Snounou, RN Davidson, Z Toossi
Journal of Experimental Medicine 1999
Synthesis of oligosaccharide fragments of mannosylated lipoarabinomannan from Mycobacterium tuberculosis
V Subramaniam, TL Lowary
Tetrahedron 1999
Structural study of the LipoMannans from Mycobacterium bovis BCG: characterisation of multiacylated forms of the phosphatidyl-myo-inositol anchor
M Gilleron, J Nigou, B Cahuzac, G Puzo
Journal of Molecular Biology 1999
Activation of NF- κ B in Mycobacterium tuberculosis – induced Interleukin-2 Receptor Expression in Mononuclear Phagocytes
KM Tchou-Wong, O Tanabe, C Chi, TA Yie, WN Rom
American journal of respiratory and critical care medicine 1999
Dysregulated Production of Interleukin-8 in Individuals Infected with Human Immunodeficiency Virus Type 1 andMycobacterium tuberculosis
JR McGhee, S Meddows-Taylor, DJ Martin, CT Tiemessen
Infection and immunity 1999
Elevated Chemokine Concentrations in Sera of Human Immunodeficiency Virus (HIV)-Seropositive and HIV-Seronegative Patients with Tuberculosis: a Possible Role for Mycobacterial Lipoarabinomannan
SH Kaufmann, NP Juffermans, A Verbon, SJ van Deventer, H van Deutekom, JT Belisle, ME Ellis, P Speelman, T van der Poll
Infection and immunity 1999
Structural definition of arabinomannans from Mycobacterium bovis BCG
J Nigou, M Gilleron, T Brando, A Vercellone, G Puzo
Glycoconjugate Journal 1999
The A and the extended V N-terminal regions of streptococcal protein I/IIf mediate the production of tumour necrosis factor alpha in the monocyte cell line THP-1
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Molecular Microbiology 1998
Differential T cell responses toMycobacterium tuberculosis ESAT6 in tuberculosis patients and healthy donors
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European Journal of Immunology 1998
ACTIVATION OF HUMAN DENDRITIC CELLS BY BACILLUS CALMETTE-GUERIN
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The Journal of Urology 1998
Differential responses of human mononuclear phagocytes to mycobacterial lipoarabinomannans: role of CD14 and the mannose receptor
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Infection and immunity 1998
Functional Activity of Alveolar and Peripheral Cells in Patients with Human Acquired Immunodeficiency Syndrome and Pulmonary Tuberculosis
MG Bonecini-Almeida, E Werneck-Barroso, PB Carvalho, CP de Moura, EF Andrade, A Hafner, CE Carvalho, JL Ho, AL Kritski, MG Morgado
Cellular Immunology 1998
Mycobacterial lipoarabinomannan: An extraordinary lipoheteroglycan with profound physiological effects
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Glycobiology 1998
Interleukin-8 and Markers of Neutrophil Degranulation in Pleural Effusions
RM Segura, J Alegre, E Varela, R Marti, JM Suriñach, J Jufresa, L Armadans, C Pascual, TO de SEVILLA
American journal of respiratory and critical care medicine 1998
The Host Immune Response to Tuberculosis
NW Schluger, WN Rom
American journal of respiratory and critical care medicine 1998
Local Immune Responses Correlate with Presentation and Outcome in Tuberculosis
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American journal of respiratory and critical care medicine 1998
Chemokines Induced by Infection of Mononuclear Phagocytes with Mycobacteria and Present in Lung Alveoli during Active Pulmonary Tuberculosis
MI Sadek, E Sada, Z Toossi, SK Schwander, EA Rich
American journal of respiratory cell and molecular biology 1998
Early Inhibition of Mycobacterial Growth by Human Alveolar Macrophages is not Due to Nitric Oxide
C Aston, WN Rom, AT Talbot, J Reibman
American journal of respiratory and critical care medicine 1998
Chemokine Production by a Human Alveolar Epithelial Cell Line in Response to Mycobacterium tuberculosis
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Infection and immunity 1998
Regulation of Macrophage Gene Expression by Mycobacterium tuberculosis : Down-Regulation of Mitochondrial Cytochrome c Oxidase
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Infection and immunity 1998
Chemokines: leucocyte recruitment and activation cytokines
DH Adams, A Rlloyd
The Lancet 1997
Early responses to infection: chemokines as mediators of inflammation
NW Schluger, WN Rom
Current Opinion in Immunology 1997
Effects of mycobacteria on regulation of apoptosis in mononuclear phagocytes
K Klingler, KM Tchou-Wong, O Brändli, C Aston, R Kim, C Chi, WN Rom
Infection and immunity 1997
Chemokine secretion by human polymorphonuclear granulocytes after stimulation with Mycobacterium tuberculosis and lipoarabinomannan
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Infection and immunity 1997
The Chemokine FactsBook
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The Chemokine FactsBook 1997
Levels of interleukin-8 in tuberculous pleurisy and the profile of immunocompetent cells in pleural and peripheral compartments
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Immunology Letters 1997
GM-CSF Gene Expression Is Normal but Protein Release Is Absent in a Patient with Pulmonary Alveolar Proteinosis
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American journal of respiratory and critical care medicine 1997
Modulation of leukocyte-endothelial cell interactions by infectious agents
KA Kelly
Bulletin de l'Institut Pasteur 1997
The Phosphatidyl- myo -inositol Anchor of the Lipoarabinomannans from Mycobacterium bovis Bacillus Calmette Guérin: HETEROGENEITY, STRUCTURE, AND ROLE IN THE REGULATION OF CYTOKINE SECRETION
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The Journal of biological chemistry 1997
Effects of mycobacteria on regulation of apoptosis in mononuclear phagocytes.
Klingler K, Tchou-Wong KM, Brändli O, Aston C, Kim R, Chi C, Rom WN
Infection and immunity 1997
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Molecular Aspects of Medicine 1996
Bacterial modulins: a novel class of virulence factors which cause host tissue pathology by inducing cytokine synthesis
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Microbiological reviews 1996
Immunopathology of tuberculosis: roles of macrophages and monocytes
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Infection and immunity 1996
Cytokine secretion in vivo and ex vivo following chemotherapy of Mycobacterium tuberculosis infection
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Transactions of the Royal Society of Tropical Medicine and Hygiene 1996

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