Mycobacterium tuberculosis infection is accompanied by acute and chronic inflammatory infiltrates associated with necrotizing granulomas in lung tissue. The cellular infiltrate is characterized by inflammatory cells which include neutrophils, lymphocytes, and macrophages. In animal and in vitro models of mycobacterial infection, cytokines including tumor necrosis factor-alpha (TNF-alpha), interferon gamma (IFN-gamma), and interleukin-1 beta (IL-1 beta) participate in granulomatous inflammation. We hypothesized that interleukin-3, a potent chemoattractant for neutrophils and lymphocytes, could be released by activated alveolar macrophages after exposure to M. tuberculosis or its components and contribute to granulomatous lung inflammation. A quantitative immunoassay revealed that IL-8 protein release was significantly elevated in supernatants of macrophages and in lavage fluid obtained from patients with pulmonary tuberculosis compared to normal controls. In addition, Northern blots demonstrated striking up-regulation of IL-8 mRNA in macrophages from these patients. M. tuberculosis and its cell wall components lipoarabinomannan (LAM), lipomannan (LM), and phosphoinositolmannoside (PIM) stimulated IL-8 protein release and mRNA expression in vitro from alveolar macrophages, but deacylated LAM did not. Neutralizing antibodies to TNF-alpha and/or IL-1-alpha and beta blocked 83% of the stimulation. IL-8 synthesis and release is an early response of macrophages after phagocytosis of M. tuberculosis. Its production serves to attract both acute and chronic inflammatory cells of active infection and thus participates in the process of containment of the pathogen.
Y Zhang, M Broser, H Cohen, M Bodkin, K Law, J Reibman, W N Rom
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2002 | |
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M Engele, E Stöβel, K Castiglione, N Schwerdtner, M Wagner, P Bölcskei, M Röllinghoff, S Stenger |
Journal of immunology (Baltimore, Md. : 1950) | 2002 |
Mycobacterium bovis BCG Vaccination Augments Interleukin-8 mRNA Expression and Protein Production in Guinea Pig Alveolar Macrophages Infected with Mycobacterium tuberculosis
MJ Lyons, T Yoshimura, DN McMurray |
Infection and immunity | 2002 |
Interleukin-8 Secretion from Mycobacterium tuberculosis -Infected Monocytes Is Regulated by Protein Tyrosine Kinases but Not by ERK1/2 or p38 Mitogen-Activated Protein Kinases
C Ameixa, JS Friedland |
Infection and immunity | 2002 |
Mycobacterium avium Complex Promotes Recruitment of Monocyte Hosts for HIV-1 and Bacteria
H Hale-Donze, T Greenwell-Wild, D Mizel, TM Doherty, D Chatterjee, JM Orenstein, SM Wahl |
Journal of immunology (Baltimore, Md. : 1950) | 2002 |
Regulation of Interleukin-8 by Interleukin-10 and Transforming Growth Factor β in Human Monocytes Infected with Mycobacterium bovis
P Méndez-Samperio, E García, A Vázquez, J Palma |
Clinical and vaccine immunology : CVI | 2002 |
Increased Mycobacterium tuberculosis growth in HIV-1-infected human macrophages: role of tumour necrosis factor-alpha
FG Imperiali, A Zaninoni, LL Maestra, P Tarsia, F Blasi, W Barcellini |
Clinical & Experimental Immunology | 2001 |
Priming of human neutrophils by mycobacterial lipoarabinomannans: role of granule mobilisation
J Fäldt, C Dahlgren, M Ridell, A Karlsson |
Microbes and Infection | 2001 |
Roles of Intracellular Calcium and NF-κB in the Bacillus Calmette- Guérin-Induced Secretion of Interleukin-8 from Human Monocytes
P Méndez-Samperio, J Palma, A Vázquez |
Cellular Immunology | 2001 |
Inflammation
JA Hunt |
Encyclopedia of Materials Science and Technology | 2001 |
Expression of IL-15 in inflammatory pulmonary diseases
S Muro, R Taha, A Tsicopoulos, R Olivenstein, AB Tonnel, P Christodoulopoulos, B Wallaert, Q Hamid |
Journal of Allergy and Clinical Immunology | 2001 |
Mononuclear cells ingest E. coli opsonized by investigational intravenous immunoglobulin preparations in the absence of complement more efficiently than polymorphonuclear phagocytes
C Tinguely, M Schaller, UE Nydegger |
Transfusion and Apheresis Science | 2001 |
Activation of Peripheral Blood Neutrophils from Patients with Active Advanced Tuberculosis
M Alemán, M Beigier-Bompadre, C Borghetti, S la Barrera, E Abbate, M Isturiz, MC Sasiain |
Clinical Immunology | 2001 |
Human Immunodeficiency Virus–Seronegative Adults with Extrapulmonary Tuberculosis Have Abnormal Innate Immune Responses
TR Sterling, SE Dorman, RE Chaisson, L Ding, J Hackman, K Moore, SM Holland |
Clinical Infectious Diseases | 2001 |
Plasma and BAL Fluid Concentrations of Antimicrobial Peptides in Patients With Mycobacterium avium- intracellulare Infection
J Ashitani, H Mukae, T Hiratsuka, M Nakazato, K Kumamoto, S Matsukura |
Chest | 2001 |
Glycoscience: Chemistry and Chemical Biology I–III
BO Fraser-Reid, K Tatsuta, J Thiem |
2001 | |
Down-Regulation of Interleukin-8 Secretion from Mycobacterium tuberculosis -Infected Monocytes by Interleukin-4 and -10 but Not by Interleukin-13
JM Mansfield, C Ameixa, JS Friedland |
Infection and immunity | 2001 |
Induction of Inducible Nitric Oxide Synthase-NO· by Lipoarabinomannan of Mycobacterium tuberculosis Is Mediated by MEK1-ERK, MKK7-JNK, and NF-κB Signaling Pathways
JT Barbieri, ED Chan, KR Morris, JT Belisle, P Hill, LK Remigio, PJ Brennan, DW Riches |
Infection and immunity | 2001 |
URINARY INTERLEUKIN-8 AND 18 PREDICT THE RESPONSE OF SUPERFICIAL BLADDER CANCER TO INTRAVESICAL THERAPY WITH BACILLUS CALMETTE-GUERIN
GN Thalmann, A Sermier, C Rentsch, K M??Hrle, MG Cecchini, UE Studer |
The Journal of Urology | 2000 |
Hut Lung
JA Gold, J Jagirdar, JG Hay, DJ Addrizzo-Harris, DP Naidich, WN Rom |
Medicine | 2000 |
Clinical investigation of pulmonary Mycobacterium avium complex infection in human T lymphotrophic virus type I carriers
W Matsuyama, A Mizoguchi, F Iwami, Y Koreeda, J Wakimoto, H Kanazawa, S Mori, M Kawabata, H Fukunaga, M Osame |
Thorax | 2000 |
Differential tolerance induction by lipoarabinomannan and lipopolysaccharide in human macrophages
DD Riedel, SH Kaufmann |
Microbes and Infection | 2000 |
Mycobacterial Lipoarabinomannan Induces an Inflammatory Response in the Mouse Lung: A Role for Interleukin-1
NP Juffermans, A Verbon, JT Belisle, PJ Hill, P Speelman, SA van DEVENTER, TO van der POLL |
American journal of respiratory and critical care medicine | 2000 |
LPS and LAM activation of the U373 astrocytoma cell line: Differential requirement for CD14
SL Orr, P Tobias |
Journal of Endotoxin Research | 2000 |
Mycobacterium tuberculosis H37Rv Parietal and Cellular Lipoarabinomannans: CHARACTERIZATION OF THE ACYL- AND GLYCO-FORMS
M Gilleron, L Bala, T Brando, A Vercellone, G Puzo |
The Journal of biological chemistry | 2000 |
Development of an Asthma Vaccine: Research into BCG
CB Scanga, GL Gros |
Drugs | 2000 |
CRL-1072 Enhances Antimycobacterial Activity of Human Macrophages Through Interleukin-8
C Jagannath, S Pai, JK Actor, RL Hunter |
Journal of Interferon & Cytokine Research | 1999 |
Influence of polymorphism in the genes for the interleukin (IL)-1 receptor antagonist and IL-1beta on tuberculosis
RJ Wilkinson, P Patel, M Llewelyn, CS Hirsch, G Pasvol, G Snounou, RN Davidson, Z Toossi |
Journal of Experimental Medicine | 1999 |
Lipoarabinomannans: characterization of the multiacylated forms of the phosphatidyl-myo-inositol anchor by NMR spectroscopy
J Nigou, M Gilleron, G Puzo |
Biochemical Journal | 1999 |
Roles of lipoarabinomannan in the pathogenesis of tuberculosis
GR Strohmeier, MJ Fenton |
Microbes and Infection | 1999 |
Influence of Polymorphism in the Genes for the Interleukin (IL)-1 Receptor Antagonist and IL-1 on Tuberculosis
RJ Wilkinson, P Patel, M Llewelyn, CS Hirsch, G Pasvol, G Snounou, RN Davidson, Z Toossi |
Journal of Experimental Medicine | 1999 |
Synthesis of oligosaccharide fragments of mannosylated lipoarabinomannan from Mycobacterium tuberculosis
V Subramaniam, TL Lowary |
Tetrahedron | 1999 |
Structural study of the LipoMannans from Mycobacterium bovis BCG: characterisation of multiacylated forms of the phosphatidyl-myo-inositol anchor
M Gilleron, J Nigou, B Cahuzac, G Puzo |
Journal of Molecular Biology | 1999 |
Activation of NF- κ B in Mycobacterium tuberculosis – induced Interleukin-2 Receptor Expression in Mononuclear Phagocytes
KM Tchou-Wong, O Tanabe, C Chi, TA Yie, WN Rom |
American journal of respiratory and critical care medicine | 1999 |
Dysregulated Production of Interleukin-8 in Individuals Infected with Human Immunodeficiency Virus Type 1 andMycobacterium tuberculosis
JR McGhee, S Meddows-Taylor, DJ Martin, CT Tiemessen |
Infection and immunity | 1999 |
Elevated Chemokine Concentrations in Sera of Human Immunodeficiency Virus (HIV)-Seropositive and HIV-Seronegative Patients with Tuberculosis: a Possible Role for Mycobacterial Lipoarabinomannan
SH Kaufmann, NP Juffermans, A Verbon, SJ van Deventer, H van Deutekom, JT Belisle, ME Ellis, P Speelman, T van der Poll |
Infection and immunity | 1999 |
Structural definition of arabinomannans from Mycobacterium bovis BCG
J Nigou, M Gilleron, T Brando, A Vercellone, G Puzo |
Glycoconjugate Journal | 1999 |
The A and the extended V N-terminal regions of streptococcal protein I/IIf mediate the production of tumour necrosis factor alpha in the monocyte cell line THP-1
C Chatenay-Rivauday, I Yamodo, MA Sciotti, JA Ogier, JP Klein |
Molecular Microbiology | 1998 |
Differential T cell responses toMycobacterium tuberculosis ESAT6 in tuberculosis patients and healthy donors
T Ulrichs, ME Munk, H Mollenkopf, S Behr-Perst, R Colangeli, ML Gennaro, SH Kaufmann |
European Journal of Immunology | 1998 |
ACTIVATION OF HUMAN DENDRITIC CELLS BY BACILLUS CALMETTE-GUERIN
R Ramoner, C Rieser, M Herold, H Klocker, G Bartsch, A Stenzl, M Thurnher |
The Journal of Urology | 1998 |
Differential responses of human mononuclear phagocytes to mycobacterial lipoarabinomannans: role of CD14 and the mannose receptor
J Bernardo, AM Billingslea, RL Blumenthal, KF Seetoo, ER Simons, MJ Fenton |
Infection and immunity | 1998 |
Functional Activity of Alveolar and Peripheral Cells in Patients with Human Acquired Immunodeficiency Syndrome and Pulmonary Tuberculosis
MG Bonecini-Almeida, E Werneck-Barroso, PB Carvalho, CP de Moura, EF Andrade, A Hafner, CE Carvalho, JL Ho, AL Kritski, MG Morgado |
Cellular Immunology | 1998 |
Mycobacterial lipoarabinomannan: An extraordinary lipoheteroglycan with profound physiological effects
D Chatterjee, KH Khoo |
Glycobiology | 1998 |
Interleukin-8 and Markers of Neutrophil Degranulation in Pleural Effusions
RM Segura, J Alegre, E Varela, R Marti, JM Suriñach, J Jufresa, L Armadans, C Pascual, TO de SEVILLA |
American journal of respiratory and critical care medicine | 1998 |
The Host Immune Response to Tuberculosis
NW Schluger, WN Rom |
American journal of respiratory and critical care medicine | 1998 |
Local Immune Responses Correlate with Presentation and Outcome in Tuberculosis
R Condos, WN Rom, YM Liu, NW Schluger |
American journal of respiratory and critical care medicine | 1998 |
Chemokines Induced by Infection of Mononuclear Phagocytes with Mycobacteria and Present in Lung Alveoli during Active Pulmonary Tuberculosis
MI Sadek, E Sada, Z Toossi, SK Schwander, EA Rich |
American journal of respiratory cell and molecular biology | 1998 |
Early Inhibition of Mycobacterial Growth by Human Alveolar Macrophages is not Due to Nitric Oxide
C Aston, WN Rom, AT Talbot, J Reibman |
American journal of respiratory and critical care medicine | 1998 |
Chemokine Production by a Human Alveolar Epithelial Cell Line in Response to Mycobacterium tuberculosis
Y Lin, M Zhang, PF Barnes |
Infection and immunity | 1998 |
Regulation of Macrophage Gene Expression by Mycobacterium tuberculosis : Down-Regulation of Mitochondrial Cytochrome c Oxidase
S Ragno, I Estrada-Garcia, R Butler, MJ Colston |
Infection and immunity | 1998 |
Chemokines: leucocyte recruitment and activation cytokines
DH Adams, A Rlloyd |
The Lancet | 1997 |
Early responses to infection: chemokines as mediators of inflammation
NW Schluger, WN Rom |
Current Opinion in Immunology | 1997 |
Effects of mycobacteria on regulation of apoptosis in mononuclear phagocytes
K Klingler, KM Tchou-Wong, O Brändli, C Aston, R Kim, C Chi, WN Rom |
Infection and immunity | 1997 |
Chemokine secretion by human polymorphonuclear granulocytes after stimulation with Mycobacterium tuberculosis and lipoarabinomannan
DD Riedel, SH Kaufmann |
Infection and immunity | 1997 |
The Chemokine FactsBook
K Vaddi, M Keller, RC Newton |
The Chemokine FactsBook | 1997 |
Levels of interleukin-8 in tuberculous pleurisy and the profile of immunocompetent cells in pleural and peripheral compartments
D Dlugovitzky, L Rateni, A Torres-Morales, J Ruiz-Silva, R Piñesky, B Canosa, O Molteni, O Bottasso |
Immunology Letters | 1997 |
GM-CSF Gene Expression Is Normal but Protein Release Is Absent in a Patient with Pulmonary Alveolar Proteinosis
KM Tchou-Wong, TJ Harkin, C Chi, M Bodkin, WN Rom |
American journal of respiratory and critical care medicine | 1997 |
Modulation of leukocyte-endothelial cell interactions by infectious agents
KA Kelly |
Bulletin de l'Institut Pasteur | 1997 |
The Phosphatidyl- myo -inositol Anchor of the Lipoarabinomannans from Mycobacterium bovis Bacillus Calmette Guérin: HETEROGENEITY, STRUCTURE, AND ROLE IN THE REGULATION OF CYTOKINE SECRETION
J Nigou, M Gilleron, B Cahuzac, JD Bounéry, M Herold, M Thurnher, G Puzo |
The Journal of biological chemistry | 1997 |
Effects of mycobacteria on regulation of apoptosis in mononuclear phagocytes.
Klingler K, Tchou-Wong KM, Brändli O, Aston C, Kim R, Chi C, Rom WN |
Infection and immunity | 1997 |
The molecular basis of mycobacterial infection
MJ Colston |
Molecular Aspects of Medicine | 1996 |
Bacterial modulins: a novel class of virulence factors which cause host tissue pathology by inducing cytokine synthesis
B Henderson, S Poole, M Wilson |
Microbiological reviews | 1996 |
Immunopathology of tuberculosis: roles of macrophages and monocytes
MJ Fenton, MW Vermeulen |
Infection and immunity | 1996 |
Cytokine secretion in vivo and ex vivo following chemotherapy of Mycobacterium tuberculosis infection
JS Friedland, JC Hartley, CG Hartley, RJ Shattock, GE Griffin |
Transactions of the Royal Society of Tropical Medicine and Hygiene | 1996 |