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Research Article Free access | 10.1172/JCI117613

Evidence for deactivation of both ectosolic and cytosolic 5'-nucleotidase by adenosine A1 receptor activation in the rat cardiomyocytes.

M Kitakaze, M Hori, T Minamino, S Takashima, K Komamura, K Node, T Kurihara, T Morioka, H Sato, and M Inoue

First Department of Medicine, Osaka University School of Medicine, Japan.

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First Department of Medicine, Osaka University School of Medicine, Japan.

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First Department of Medicine, Osaka University School of Medicine, Japan.

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First Department of Medicine, Osaka University School of Medicine, Japan.

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First Department of Medicine, Osaka University School of Medicine, Japan.

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First Department of Medicine, Osaka University School of Medicine, Japan.

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First Department of Medicine, Osaka University School of Medicine, Japan.

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First Department of Medicine, Osaka University School of Medicine, Japan.

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First Department of Medicine, Osaka University School of Medicine, Japan.

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First Department of Medicine, Osaka University School of Medicine, Japan.

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Published December 1, 1994 - More info

Published in Volume 94, Issue 6 on December 1, 1994
J Clin Invest. 1994;94(6):2451–2456. https://doi.org/10.1172/JCI117613.
© 1994 The American Society for Clinical Investigation
Published December 1, 1994 - Version history
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Abstract

Adenosine, an important regulator of many cardiac functions, is produced by ectosolic and cytosolic 5'-nucleotidase. The activity of these enzymes is influenced by several ischemia-sensitive metabolic factors, e.g., ATP, ADP, H+, and inorganic phosphate. However, there is no clear evidence that adenosine itself affects 5'-nucleotidase activity. This study tested whether adenosine decreases the activity of ectosolic and cytosolic 5'-nucleotidase. Cardiomyocytes were isolated from adult male Wistar rats and suspended in the modified Hepes-Tyrode buffer solution. After stabilization, isolated cardiomyocytes were incubated with and without adenosine (10(-9) - 10(-4) M). Ectosolic and cytosolic 5'-nucleotidase activity was decreased by exogenous adenosine (ectosolic 5'-nucleotidase activity, 20.6 +/- 2.3 vs. 8.6 +/- 1.6 mumol/min per 10(6) cells [P < 0.05]; cytosolic 5'-nucleotidase activity, 2.47 +/- 0.58 vs. 1.61 +/- 0.54 mumol/min per 10(6) cells [P < 0.05] at 10(-6) M adenosine) after 30 min. The decrease in ectosolic and cytosolic 5'-nucleotidase activity was inhibited by 8-phenyltheophylline and pertussis toxin, and was mimicked by N6-cyclohexyladenosine, an adenosine A1 receptor agonist. Neither CGS21680C, and A2 receptor agonist, nor cycloheximide deactivated ectosolic and cytosolic 5'-nucleotidase. Thus, we conclude that activation of adenosine A1 receptors is coupled to Gi proteins and attenuates ectosolic and cytosolic 5'-nucleotidase activity in rat cardiomyocytes.

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