Advertisement

Research Article Free access | 10.1172/JCI117256

In situ glucose uptake and glucokinase activity of pancreatic islets in diabetic and obese rodents.

Y Liang, S Bonner-Weir, Y J Wu, C D Berdanier, D K Berner, S Efrat, and F M Matschinsky

Department of Biochemistry and Biophysics, University of Pennyslvania, Philadelphia 19104.

Find articles by Liang, Y. in: JCI | PubMed | Google Scholar

Department of Biochemistry and Biophysics, University of Pennyslvania, Philadelphia 19104.

Find articles by Bonner-Weir, S. in: JCI | PubMed | Google Scholar

Department of Biochemistry and Biophysics, University of Pennyslvania, Philadelphia 19104.

Find articles by Wu, Y. in: JCI | PubMed | Google Scholar

Department of Biochemistry and Biophysics, University of Pennyslvania, Philadelphia 19104.

Find articles by Berdanier, C. in: JCI | PubMed | Google Scholar

Department of Biochemistry and Biophysics, University of Pennyslvania, Philadelphia 19104.

Find articles by Berner, D. in: JCI | PubMed | Google Scholar

Department of Biochemistry and Biophysics, University of Pennyslvania, Philadelphia 19104.

Find articles by Efrat, S. in: JCI | PubMed | Google Scholar

Department of Biochemistry and Biophysics, University of Pennyslvania, Philadelphia 19104.

Find articles by Matschinsky, F. in: JCI | PubMed | Google Scholar

Published June 1, 1994 - More info

Published in Volume 93, Issue 6 on June 1, 1994
J Clin Invest. 1994;93(6):2473–2481. https://doi.org/10.1172/JCI117256.
© 1994 The American Society for Clinical Investigation
Published June 1, 1994 - Version history
View PDF
Abstract

The present study evaluated the involvement of glucose transport and phosphorylation in glucose-stimulated insulin release from pancreatic islets. Using quantitative histochemical techniques, we investigated basal islet glucose content, islet glucose uptake in situ during acute extreme experimental hyperglycemia, and islet glucokinase activity in several animal models of diabetes and obesity. The basal islet glucose content in anaesthetized diabetic or obese rodents was either the same or higher than that in their relevant controls. The rate of glucose uptake of islet tissue in these animals after an i.v. glucose injection was different. The db+/db+ mouse and the obese Zucker rat exhibited significantly reduced islet glucose uptake rates. RIP-cHras transgenic mice, BHE/cdb rats and partially pancreatectomized rats showed normal islet glucose uptake rates. The activity of islet glucokinase was increased to a different degree related to the blood glucose level. All five animal models of diabetes or obesity exhibited either a delay or a reduction of insulin release in response to supra maximal glucose stimulation. Our results indicate that the impairment of glucose-induced insulin release in diabetes is not consistently associated with a reduction of islet glucose uptake nor a change of glucokinase activity.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

Version history
  • Version 1 (June 1, 1994): No description
Advertisement
Advertisement