The overall objective of these studies was to determine whether IgG antibody to Pseudomonas aeruginosa would modify the acute lung and pleural injury that developed over 24 h after the instillation of 10(10) live P. aeruginosa into the distal airspaces of one lung in unanesthetized sheep. Using a quantitative experimental model to measure protein permeability across the alveolar epithelial, lung endothelial, and pleural mesothelial barriers, the effect of IgG antibody to P. aeruginosa was examined under four different experimental conditions. First, the effect of IgG antibody to P. aeruginosa in the circulation was examined by instilling 10(10) live P. aeruginosa in 5% ovine albumin in sheep that had been vaccinated. Under these conditions, the presence of circulating IgG antibody to P. aeruginosa reduced lung endothelial injury but did not modify the lung epithelial or pleural injury caused by intraalveolar P. aeruginosa. Therefore, the second experimental protocol determined the effect of instilling immune serum from a sheep that had been vaccinated so that IgG antibody to P. aeruginosa was present in both the circulation and in the airspaces along with instillation of live bacteria. Under these conditions, injury to the lung endothelium, alveolar epithelium, and pleural space was completely prevented. Therefore, the third protocol examined the protective effect of instillation of IgG antibody to P. aeruginosa in the airspaces concurrent with the live bacteria. Interestingly, intraalveolar IgG antibody to P. aeruginosa prevented all evidence of lung epithelial and pleural injury, and this effect was associated with a marked decrease in the number of viable bacteria in the lung after 24 h. Therefore, the fourth protocol examined the prophylactic effect of instillation of the specific IgG antibody to P. aeruginosa 24 h before instillation of the bacteria. With this prophylactic regimen, epithelial, endothelial, and pleural injury were prevented, and there was a significant decrease in the number of bacteria recovered from the lung. Thus, delivery of IgG antibody to P. aeruginosa the distal airspaces of the lung alone may provide a novel therapeutic approach to preventing acute pulmonary infection caused by P. aeruginosa.
J F Pittet, M A Matthay, G Pier, M Grady, J P Wiener-Kronish
Title and authors | Publication | Year |
---|---|---|
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S Zarogiannis, C Hatzoglou, I Stefanidis, M Ioannou, E Paraskeva, K Gourgoulianis, PA Molyvdas |
Clinical and Experimental Pharmacology and Physiology | 2007 |
Differential effects of sustained inflation recruitment maneuvers on alveolar epithelial and lung endothelial injury*
JA Frank, DF McAuley, JA Gutierrez, BM Daniel, L Dobbs, MA Matthay |
Critical Care Medicine | 2005 |
Role of Rho-kinase in reexpansion pulmonary edema in rabbits
M Sawafuji, A Ishizaka, M Kohno, H Koh, S Tasaka, Y Ishii, K Kobayashi |
American journal of physiology. Lung cellular and molecular physiology | 2005 |
Alveolar Response to Pseudomonas aeruginosa : Role of the Type III Secretion System
F Ader, RL Berre, K Faure, P Gosset, O Epaulard, B Toussaint, B Polack, E Nowak, NB Viget, E Kipnis, BP Guery |
Infection and immunity | 2005 |
Electron microscopic alterations of the rat's pleura after experimental haemothorax
KN Michailova |
Annals of Anatomy - Anatomischer Anzeiger | 2004 |
Protein transport across the lung epithelial barrier
KJ Kim, AB Malik |
American journal of physiology. Lung cellular and molecular physiology | 2003 |
High tidal volume ventilation induces NOS2 and impairs cAMP- dependent air space fluid clearance
JA Frank, JF Pittet, H Lee, M Godzich, MA Matthay |
American journal of physiology. Lung cellular and molecular physiology | 2003 |
Lung Epithelial Fluid Transport and the Resolution of Pulmonary Edema
MA Matthay, HG Folkesson, C Clerici |
Physiological reviews | 2002 |
Protective effects of low tidal volume ventilation in a rabbit model of Pseudomonas aeruginosa-induced acute lung injury
RH Savel, EC Yao, MA Gropper |
Critical Care Medicine | 2001 |
The ovine cathelicidin SMAP29 kills ovine respiratory pathogens in vitro and in an ovine model of pulmonary infection
KA Brogden, VC Kalfa, MR Ackermann, DE Palmquist, PB McCray, BF Tack |
Antimicrobial agents and chemotherapy | 2001 |
Efficacy of Low Tidal Volume Ventilation in Patients with Different Clinical Risk Factors for Acute Lung Injury and the Acute Respiratory Distress Syndrome
MD Eisner, T Thompson, LD Hudson, JM Luce, D Hayden, D Schoenfeld, MA Matthay |
American journal of respiratory and critical care medicine | 2001 |
PARTITIONING LUNG AND PLASMA PROTEINS: CIRCULATING SURFACTANT PROTEINS AS BIOMARKERS OF ALVEOLOCAPILLARY PERMEABILITY
IR Doyle, TE Nicholas, AD Bersten |
Clinical and Experimental Pharmacology and Physiology | 1999 |
The American–European Consensus Conference on ARDS, Part 2: Ventilatory, Pharmacologic, Supportive Therapy, Study Design Strategies, and Issues Related to Recovery and Remodeling
A Artigas, GR Bernard, J Carlet, D Dreyfuss, L Gattinoni, L Hudson, M Lamy, JJ Marini, MA Matthay, MR Pinsky, R Spragg, PM Suter |
American journal of respiratory and critical care medicine | 1998 |
The American-European consensus conference on ARDS, Part 2: Ventilatory, pharmacologic, supportive therapy, study design strategies and issues related to recovery and remodeling
A Artigas, GR Bernard, J Carlet, D Dreyfuss, L Gattinoni, L Hudson, M Lamy, JJ Marini, MA Matthay, MR Pinsky, R Spragg, PM Suter |
Intensive Care Medicine | 1998 |
Intraluminal Water Increases Expression of Plasmid DNA in Rat Lung
T Sawa, H Miyazaki, JF Pittet, JH Widdicombe, MA Gropper, S Hashimoto, DJ Conrad, HG Folkesson, R Debs, JR Forsayeth, B Fox, JP Wiener-Kronish |
Human Gene Therapy | 1996 |
Potential of preventing Pseudomonas aeruginosa lung infections in cystic fibrosis patients: Experimental studies in animals
HK Johansen |
APMIS | 1996 |
Protection by intratracheal immune globulins against Pseudomanas aeruginosa pneumonia in mice
CM Paranavitana, JR Boyce, T Burnstein |
Serodiagnosis and Immunotherapy in Infectious Disease | 1996 |
Cystic fibrosis epithelial cells have a receptor for pathogenic bacteria on their apical surface
L Imundo, J Barasch, A Prince, Q Al-Awqati |
Proceedings of the National Academy of Sciences | 1995 |