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Citations to this article

Renal acid excretion and intracellular pH in salt-sensitive genetic hypertension.
D C Batlle, … , A Saleh, C Gutterman
D C Batlle, … , A Saleh, C Gutterman
Published May 1, 1993
Citation Information: J Clin Invest. 1993;91(5):2178-2184. https://doi.org/10.1172/JCI116444.
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Research Article

Renal acid excretion and intracellular pH in salt-sensitive genetic hypertension.

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Abstract

Acid-base status and renal acid excretion were studied in the Dahl/Rapp salt-sensitive (S) rat and its genetically salt-resistant counterpart (R). S rats developed hypertension while on a very high salt diet (8%) and while on a more physiological salt diet (1%) and remained normotensive while on a very low salt diet (0.08%). Under the high salt diet, intracellular pH measured in freshly isolated thymic lymphocytes using 2',7'-bis (carboxyethyl)-5 (6)-carboxyfluorescein acetomethyl ester, a pH-sensitive dye, was lower in S than in R rats both when measured in the presence of HCO3/CO2 (7.32 +/- 0.02 vs. 7.38 +/- 0.02, respectively, P < 0.05) and in its absence (7.18 +/- 0.04 vs. 7.27 +/- 0.02, respectively, P < 0.05). Under the high salt diet, net acid excretion was higher in S than R rats (1,777 +/- 111 vs. 1,017 +/- 73 muEq/24 h per 100 g body wt, respectively, P < 0.001), and this difference was due to higher rates of both titratable acid and ammonium excretion. Directionally similar differences in intracellular pH and net acid excretion between S and R rats were also observed in salt-restricted animals. In S and R rats placed on a normal salt intake (1%) and strictly pair-fed to control food intake as a determinant of dietary acid, net acid excretion was also higher in S than in R rats (562 +/- 27 vs. 329 +/- 21 muEq/24 h per 100 g, respectively, P < 0.01). No significant difference in either blood pH or bicarbonate levels were found between S and R rats on either the 0.08%, 1%, or 8% salt diets. We conclude that renal acid excretion is augmented in the salt-sensitive Dahl/Rapp rat. Enhanced renal acid excretion may be a marker of increased acid production by cells from subjects with salt-sensitive hypertension.

Authors

D C Batlle, A M Sharma, M W Alsheikha, M Sobrero, A Saleh, C Gutterman

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Total citations by year

Year: 2021 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2003 2001 2000 1999 1998 1997 1996 1994 1993 Total
Citations: 1 2 1 1 2 1 2 1 2 1 2 1 3 1 1 1 2 1 1 1 2 3 4 1 38
Citation information
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Citations to this article (39)

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International Journal of Clinical & Laboratory Research 2000
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