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Article has an altmetric score of 3

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Referenced in 5 patents
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Research Article Free access | 10.1172/JCI115967

Serum neutralizing antibody response to the vacuolating cytotoxin of Helicobacter pylori.

T L Cover, P Cao, U K Murthy, M S Sipple, and M J Blaser

Department of Veterans Affairs Medical Center, Nashville, Tennessee 37232.

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Department of Veterans Affairs Medical Center, Nashville, Tennessee 37232.

Find articles by Cao, P. in: JCI | PubMed | Google Scholar

Department of Veterans Affairs Medical Center, Nashville, Tennessee 37232.

Find articles by Murthy, U. in: JCI | PubMed | Google Scholar

Department of Veterans Affairs Medical Center, Nashville, Tennessee 37232.

Find articles by Sipple, M. in: JCI | PubMed | Google Scholar

Department of Veterans Affairs Medical Center, Nashville, Tennessee 37232.

Find articles by Blaser, M. in: JCI | PubMed | Google Scholar

Published September 1, 1992 - More info

Published in Volume 90, Issue 3 on September 1, 1992
J Clin Invest. 1992;90(3):913–918. https://doi.org/10.1172/JCI115967.
© 1992 The American Society for Clinical Investigation
Published September 1, 1992 - Version history
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Abstract

Approximately 50% of Helicobacter pylori isolates produce a cytotoxin in vitro that induces vacuolation of eukaryotic cells. To determine the in vivo relevance of this phenomenon, we sought to detect cytotoxin-neutralizing antibodies in sera from H. pylori-infected persons. As a group, sera from 29 H. pylori-infected patients neutralized the activity of the purified cytotoxin to a significantly greater extent than sera from 24 uninfected persons (P = 0.007). The cytotoxin neutralizing activity in sera from H. pylori-infected persons was mediated predominantly by the purified IgG fraction. Sera from H. pylori-infected persons neutralized the cytotoxins produced by multiple H. pylori strains, but failed to neutralize trimethylamine-induced cell vacuolation. Neutralization of cytotoxin activity by human or immune rabbit sera was associated with immunoblot IgG recognition of an 87-kD H. pylori protein. Similarly, neutralization of the toxin by sera was associated with IgG recognition of the purified cytotoxin in an enzyme-linked immunosorbent assay (P less than 0.0001). The presence of cytotoxin-neutralizing antibodies in sera from H. pylori-infected persons indicates that the cytotoxin is synthesized in vivo.

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Referenced in 5 patents
18 readers on Mendeley
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