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Article has an altmetric score of 3

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Referenced in 6 patents
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Research Article Free access | 10.1172/JCI115939

The tissue distribution of tumor necrosis factor biosynthesis during endotoxemia.

B P Giroir, J H Johnson, T Brown, G L Allen, and B Beutler

Howard Hughes Medical Institute, Southwestern Medical Center, Dallas, Texas 75235.

Find articles by Giroir, B. in: JCI | PubMed | Google Scholar

Howard Hughes Medical Institute, Southwestern Medical Center, Dallas, Texas 75235.

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Howard Hughes Medical Institute, Southwestern Medical Center, Dallas, Texas 75235.

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Howard Hughes Medical Institute, Southwestern Medical Center, Dallas, Texas 75235.

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Howard Hughes Medical Institute, Southwestern Medical Center, Dallas, Texas 75235.

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Published September 1, 1992 - More info

Published in Volume 90, Issue 3 on September 1, 1992
J Clin Invest. 1992;90(3):693–698. https://doi.org/10.1172/JCI115939.
© 1992 The American Society for Clinical Investigation
Published September 1, 1992 - Version history
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Abstract

Tumor necrosis factor (TNF) is a protein hormone implicated in the development of septic shock and other pathologic states. However, complexities inherent in detecting TNF synthesis by individual tissues have left the precise origins of this protein undefined. In addition, the possibility that localized TNF production may contribute to the pathogenesis of organ-specific diseases such as type I diabetes has not been explored in vivo. We have developed a transgenic mouse line bearing a reporter gene construct in which the TNF coding sequence and introns are replaced by a chloramphenicol acetyltransferase (CAT) coding sequence. In normal transgenic animals, CAT activity is expressed only in the thymus. When endotoxin is administered to the animals, CAT activity is also evident in kidney, heart, islets of Langerhans, spleen, lung, fallopian tubes, and uterus, but not in other organs. The biosynthesis of CAT in vivo correlated with tissue capacity to secrete TNF in vitro. Thus, TNF was secreted by all the tissues that expressed CAT, including lung, spleen, thymus, uterus/fallopian tubes, pancreatic islets, renal glomeruli, and cultured cardiac cells after exposure to endotoxin.

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Referenced in 6 patents
33 readers on Mendeley
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