Advertisement

Research Article Free access | 10.1172/JCI115777

Human intraovarian interleukin-1 (IL-1) system: highly compartmentalized and hormonally dependent regulation of the genes encoding IL-1, its receptor, and its receptor antagonist.

A Hurwitz, J Loukides, E Ricciarelli, L Botero, E Katz, J M McAllister, J E Garcia, R Rohan, E Y Adashi, and E R Hernandez

Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.

Find articles by Hurwitz, A. in: JCI | PubMed | Google Scholar

Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.

Find articles by Loukides, J. in: JCI | PubMed | Google Scholar

Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.

Find articles by Ricciarelli, E. in: JCI | PubMed | Google Scholar

Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.

Find articles by Botero, L. in: JCI | PubMed | Google Scholar

Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.

Find articles by Katz, E. in: JCI | PubMed | Google Scholar

Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.

Find articles by McAllister, J. in: JCI | PubMed | Google Scholar

Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.

Find articles by Garcia, J. in: JCI | PubMed | Google Scholar

Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.

Find articles by Rohan, R. in: JCI | PubMed | Google Scholar

Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.

Find articles by Adashi, E. in: JCI | PubMed | Google Scholar

Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.

Find articles by Hernandez, E. in: JCI | PubMed | Google Scholar

Published June 1, 1992 - More info

Published in Volume 89, Issue 6 on June 1, 1992
J Clin Invest. 1992;89(6):1746–1754. https://doi.org/10.1172/JCI115777.
© 1992 The American Society for Clinical Investigation
Published June 1, 1992 - Version history
View PDF
Abstract

To delineate the scope of the human intraovarian IL-1 system we used a solution hybridization/RNase protection assay to test for expression of the genes encoding IL-1, its type I receptor (IL-1R), and its receptor antagonist (IL-1RA). IL-1 transcripts were not detected in whole ovarian material from days 4 or 12 of an unstimulated menstrual cycle but transcripts (IL-1 beta much greater than IL-11 alpha) were detected in preovulatory follicular aspirates from gonadotropin-stimulated cycles. Concurrently obtained peripheral monocytes did not contain IL-1 beta transcripts but macrophage-depleted follicular aspirates did, thus implicating the granulosa cells as the site of IL-1 expression. IL-1R transcripts were detected in RNA from whole ovaries and follicular aspirates but not in RNA from peripheral monocytes. IL-1RA transcripts were detected in whole ovarian material as well as in macrophage-free follicular aspirates. Cultured human granulosa and theca cells did not contain mRNA for IL-1 beta or IL-1RA but did contain mRNA for IL-1R. Treatment of cell cultures with forskolin (25 microM) induced IL-1 beta transcripts in granulosa but not theca cells. Forskolin also increased the basal levels of IL-1R transcripts in both granulosa and theca cells but did not induce IL-RA transcripts in either cell type. Taken together, these findings reveal the existence of a complete, highly compartmentalized, hormonally dependent intraovarian IL-1 system replete with ligands, receptor, and receptor antagonist.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

Version history
  • Version 1 (June 1, 1992): No description
Advertisement
Advertisement