Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact

Citations to this article

Effects of caerulein on the apical cytoskeleton of the pancreatic acinar cell.
M S O'Konski, S J Pandol
M S O'Konski, S J Pandol
Published November 1, 1990
Citation Information: J Clin Invest. 1990;86(5):1649-1657. https://doi.org/10.1172/JCI114887.
View: Text | PDF
Research Article

Effects of caerulein on the apical cytoskeleton of the pancreatic acinar cell.

  • Text
  • PDF
Abstract

In this study experiments were performed to correlate the rate of digestive enzyme secretion to morphologic observations of the apical cytoskeleton using dispersed rat pancreatic acini with various concentrations of caerulein. Caerulein at concentrations of 10 pM to 0.1 nM stimulated increasing rates of secretion of amylase, a digestive enzyme. Greater concentrations of caerulein caused progressively less amylase secretion. Transmission electron microscopy demonstrated several characteristics of the apical cytoskeleton in untreated acini that were altered with the "inhibitory" concentrations of caerulein. In control acini and acini stimulated with concentrations of caerulein up to 0.1 nM, the micrographs reveal an apical actin network extending into microvilli, an intermediate filament band, and electron-dense structures contained in both the actin filament network and the intermediate filament band. With concentrations of caerulein greater than 0.1 nM, these structures were progressively ablated. The findings with respect to the actin filament network were confirmed with light microscopic observations of dispersed acini stained with rhodamine-phalloidin. These results indicate that caerulein has marked morphologic effects on the pancreatic acinar cell cytoskeleton and that the cytoskeletal changes may modulate the secretory response.

Authors

M S O'Konski, S J Pandol

×

Total citations by year

Year: 2024 2022 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1996 1995 1994 1993 1975 Total
Citations: 1 2 1 1 2 2 1 1 2 2 3 1 3 3 4 6 6 6 4 2 6 3 4 6 7 1 4 4 2 1 91
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article (91)

Title and authors Publication Year
Acute pancreatitis: pathogenesis and emerging therapies
Zaman S, Gorelick F
Journal of pancreatology 2024
Identification and Validation of Hub Genes in Acute Pancreatitis and Hypertriglyceridemia.
Zhang S, Liang Z, Xiang X, Liu L, Yang H, Tang G
Diabetes, metabolic syndrome and obesity : targets and therapy 2022
Assessment of exocrine pancreatic function in children and adolescents with direct and indirect testing
Hopson P, Smadi Y, Mehta V, Patel S, Mehta D, Horvath K
Frontiers in Pediatrics 2022
Critical thresholds: key to unlocking the door to the prevention and specific treatments for acute pancreatitis
SG Barreto, A Habtezion, A Gukovskaya, A Lugea, C Jeon, D Yadav, P Hegyi, V Venglovecz, R Sutton, SJ Pandol
Gut 2020
Protective effects of urocortin 2 against caerulein-induced acute pancreatitis
J Yuan, B Hasdemir, T Tan, C Chheda, J Rivier, SJ Pandol, A Bhargava, Z Rakonczay
PloS one 2019
Multimodal Transgastric Local Pancreatic Hypothermia Reduces Severity of Acute Pancreatitis in Rats and Increases Survival
C de Oliveira, B Khatua, A Bag, B El-Kurdi, K Patel, V Mishra, S Navina, VP Singh
Gastroenterology 2018
Surgical Diseases of the Pancreas and Biliary Tree
SG Barreto, JA Windsor
2018
Do Animal Models of Acute Pancreatitis Reproduce Human Disease?
FS Gorelick, MM Lerch
Cellular and Molecular Gastroenterology and Hepatology 2017
Human Pancreatic Acinar Cells
A Lugea, RT Waldron, OA Mareninova, N Shalbueva, N Deng, HY Su, DD Thomas, EK Jones, SW Messenger, J Yang, C Hu, I Gukovsky, Z Liu, GE Groblewski, AS Gukovskaya, FS Gorelick, SJ Pandol
The American Journal of Pathology 2017
Surgical Intensive Care Medicine
V Radcliff, N MacIntyre
Surgical Intensive Care Medicine 2016
Involvement of Membrane Blebbing in Immunological Disorders and Cancer
MA Khajah, YA Luqmani
Medical Principles and Practice 2015
p120 Catenin is required for normal tubulogenesis but not epithelial integrity in developing mouse pancreas
AM Hendley, E Provost, JM Bailey, YJ Wang, MH Cleveland, D Blake, RW Bittman, JC Roeser, A Maitra, AB Reynolds, SD Leach
Developmental Biology 2014
Comprehensive Physiology
SN Cheuvront, RW Kenefick
Comprehensive Physiology 2014
Proteomic analysis of apoptotic and oncotic pancreatic acinar AR42J cells treated with caerulein
J Chu, H Ji, M Lu, Z Li, X Qiao, B Sun, W Zhang, D Xue
Molecular and Cellular Biochemistry 2013
Lasting inhibition of receptor-mediated calcium oscillations in pancreatic acini by neutrophil respiratory burst – A novel mechanism for secretory blockade in acute pancreatitis?
HY Liang, ZM Song, ZJ Cui
Biochemical and Biophysical Research Communications 2013
Ryanodine receptors contribute to bile acid-induced pathological calcium signaling and pancreatitis in mice
SZ Husain, AI Orabi, KA Muili, Y Luo, S Sarwar, SM Mahmood, D Wang, R Choo-Wing, VP Singh, J Parness, M Ananthanaravanan, V Bhandari, G Perides
American journal of physiology. Gastrointestinal and liver physiology 2012
Raf-1 kinase inhibitory protein (RKIP) mediates ethanol-induced sensitization of secretagogue signaling in pancreatic acinar cells
SO Kim, KL Ives, X Wang, RA Davey, C Chao, MR Hellmich
The Journal of biological chemistry 2012
Early proteome analysis of rat pancreatic acinar AR42J cells treated with taurolithocholic acid 3-sulfate
Z Li, M Lu, J Chu, X Qiao, X Meng, B Sun, W Zhang, D Xue
Pancreatology 2012
The Exocrine Pancreas
SJ Pandol
Colloquium Series on Integrated Systems Physiology: From Molecule to Function 2011
Pancreatitis: The Acid Test
RA Liddle
Gastroenterology 2010
Current Topics in Membranes
TC Wagenknecht, Z Liu
Structure and Function of Calcium Release Channels 2010
Sleisenger and Fordtran's Gastrointestinal and Liver Disease
C Högenauer, HF Hammer
Sleisenger and Fordtran's Gastrointestinal and Liver Disease 2010
The Acinar Cell and Early Pancreatitis Responses
FS Gorelick, E Thrower
Clinical Gastroenterology and Hepatology 2009
Calpain-mediated breakdown of cytoskeletal proteins contributes to cholecystokinin-induced damage of rat pancreatic acini
H Weber, S Hühns, F Lüthen, L Jonas
International Journal of Experimental Pathology 2009
Small G proteins as key regulators of pancreatic digestive enzyme secretion
JA Williams, X Chen, ME Sabbatini
American journal of physiology. Endocrinology and metabolism 2009
Src-mediated cortactin phosphorylation regulates actin localization and injurious blebbing in acinar cells
VP Singh, MA McNiven
Molecular biology of the cell 2008
Small G proteins as key regulators of pancreatic digestive enzyme secretion
JA Williams, X Chen, ME Sabbatini
American journal of physiology. Endocrinology and metabolism 2008
Protein kinase C in the pancreatic acinar cell
F Gorelick, S Pandol, E Thrower
Journal of Gastroenterology and Hepatology 2008
Diseases of the Pancreas
HG Beger, S Matsuno, JL Cameron, BM Rau, M Sunamura, RD Schulick
2008
Pancreatic protease-activated receptors: friend and foe
F Gorelick
Gut 2007
Why Does Pancreatic Overstimulation Cause Pancreatitis?
AK Saluja, MM Lerch, PA Phillips, V Dudeja
Annual Review of Physiology 2007
Acute Pancreatitis: Bench to the Bedside
SJ Pandol, AK Saluja, CW Imrie, PA Banks
Gastroenterology 2007
Selective cyclooxygenase-2 inhibitor ameliorates cholecystokinin-octapeptide-induced acute pancreatitis in rats
SW Seo, WS Jung, TG Piao, SH Hong, KJ Yun, RK Park, MK Shin, HJ Song, SJ Park
World journal of gastroenterology : WJG 2007
Cyclic AMP-dependent protein kinase and Epac mediate cyclic AMP responses in pancreatic acini
A Chaudhuri, SZ Husain, TR Kolodecik, WM Grant, FS Gorelick
AJP Gastrointestinal and Liver Physiology 2007
Supramaximal CCK-58 does not induce pancreatitis in the rat: role of pancreatic water secretion
M Yamamoto, JR Reeve, GM Green
AJP Gastrointestinal and Liver Physiology 2007
Suppression of transforming growth factor beta signalling aborts caerulein induced pancreatitis and eliminates restricted stimulation at high caerulein concentrations
S Wildi, J Kleeff, J Mayerle, A Zimmermann, EP Böttinger, L Wakefield, MW Büchler, H Friess, M Korc
Gut 2006
Molecular mechanisms of lacrimal acinar secretory vesicle exocytosis
K Wu, GV Jerdeva, SR da Costa, E Sou, JE Schechter, SF Hamm-Alvarez
Experimental Eye Research 2006
Long-term Ethanol Consumption Alters Pancreatic Gene Expression in Rats
CH Kubisch, I Gukovsky, A Lugea, SJ Pandol, R Kuick, DE Misek, SM Hanash, CD Logsdon
Pancreas 2006
PKC signaling in pancreatic acinar cell
A SATOH, T SHIMOSEGAWA
Suizo 2006
Physiology of the Gastrointestinal Tract
DJ Drucker
Physiology of the Gastrointestinal Tract 2006
Effects of pancreatic duct ligation on pancreatic response to bombesin
T Otani, A Matsukura, T Takamoto, Y Seyama, Y Shimizu, M Shinomiya, H Usui, FS Gorelick, M Makuuchi
AJP Gastrointestinal and Liver Physiology 2006
Tumor Necrosis Factor-α Mediates Pancreatitis Responses in Acinar Cells via Protein Kinase C and Proline-Rich Tyrosine Kinase 2
A Satoh, AS Gukovskaya, M Edderkaoui, MS Daghighian, JR Reeve, T Shimosegawa, SJ Pandol
Gastroenterology 2005
Mechanisms of Acinar Cell Injury in Acute Pancreatitis
MG Raraty, JA Murphy, E McLoughlin, D Smith, D Criddle, R Sutton
SCAND J SURG 2005
Effects of increased intracellular cAMP on carbachol-stimulated zymogen activation, secretion, and injury in the pancreatic acinar cell
A Chaudhuri, TR Kolodecik, FS Gorelick
AJP Gastrointestinal and Liver Physiology 2005
Secretin differentially sensitizes rat pancreatic acini to the effects of supramaximal stimulation with caerulein
G Perides, A Sharma, A Gopal, X Tao, K Dwyer, B Ligon, ML Steer
AJP Gastrointestinal and Liver Physiology 2005
Rho and Rac promote acinar morphological changes, actin reorganization, and amylase secretion
Y Bi, SL Page, JA Williams
AJP Gastrointestinal and Liver Physiology 2005
A role for Rho and Rac in secretagogue-induced amylase release by pancreatic acini
Y Bi, JA Williams
American journal of physiology. Cell physiology 2005
Ethanol toxicity in pancreatic acinar cells: mediation by nonoxidative fatty acid metabolites
DN Criddle, MG Raraty, JP Neoptolemos, AV Tepikin, OH Petersen, R Sutton
Proceedings of the National Academy of Sciences 2004
Disruption of Paracellular Sealing is an Early Event in Acute Caerulein-Pancreatitis
M Schmitt, H Klonowski-Stumpe, M Eckert, R Lüthen, D Häussinger
Pancreas 2004
Overexpression of heat shock protein Hsp27 protects against cerulein-induced pancreatitis
C Kubisch, MJ Dimagno, AB Tietz, MJ Welsh, SA Ernst, B Brandt-Nedelev, J Diebold, AC Wagner, B Göke, JA Williams, C Schäfer
Gastroenterology 2004
Actin overexpression parallels severity of pancreatic injury
B Zhong, MB Omary
Experimental Cell Research 2004
Signal transduction, calcium and acute pancreatitis
R Sutton, D Criddle, MG Raraty, A Tepikin, JP Neoptolemos, OH Petersen
Pancreatology 2003
CCK-A receptor activates RhoA through Gα 12/13 in NIH3T3 cells
SL le Page, Y Bi, JA Williams
American journal of physiology. Cell physiology 2003
Keratin binding to 14-3-3 proteins modulates keratin filaments and hepatocyte mitotic progression
NO Ku, S Michie, EZ Resurreccion, RL Broome, MB Omary
Proceedings of the National Academy of Sciences 2002
Caspase 8-mediated cleavage of plectin precedes F-actin breakdown in acinar cells during pancreatitis
M Beil, J Leser, MP Lutz, A Gukovskaya, T Seufferlein, G Lynch, SJ Pandol, G Adler
AJP Gastrointestinal and Liver Physiology 2002
Small GTP-binding Protein TC10 Differentially Regulates Two Distinct Populations of Filamentous Actin in 3T3L1 Adipocytes
M Kanzaki, RT Watson, JC Hou, M Stamnes, AR Saltiel, JE Pessin, D Drubin
Molecular biology of the cell 2002
Serine protease inhibitors and F-actin redistribution
MO Blackstone
Gastroenterology 2002
Lacrimal Gland, Tear Film, and Dry Eye Syndromes 3
DA Sullivan, ME Stern, K Tsubota, DA Dartt, RM Sullivan, BB Bromberg
Lacrimal Gland, Tear Film, and Dry Eye Syndromes 3 2002
Effects of reactive oxygen species on actin filament polymerisation and amylase secretion in mouse pancreatic acinar cells
JA Rosado, A González, GM Salido, JA Pariente
Cellular Signalling 2002
Arginine induced acute pancreatitis alters the actin cytoskeleton and increases heat shock protein expression in rat pancreatic acinar cells
M Tashiro, C Schäfer, H Yao, SA Ernst, JA Williams
Gut 2001
Serine Protease Inhibitor Causes F-Actin Redistribution and Inhibition of Calcium-Mediated Secretion in Pancreatic Acini
VP Singh, AK Saluja, L Bhagat, AJ Hietaranta, A Song, A Mykoniatis, GJ van Acker, ML Steer
Gastroenterology 2001
Insulin-stimulated GLUT4 Translocation in Adipocytes Is Dependent upon Cortical Actin Remodeling
M Kanzaki, JE Pessin
The Journal of biological chemistry 2001
Actin coating of secretory granules during regulated exocytosis correlates with the release of rab3D
JA Valentijn, K Valentijn, LM Pastore, JD Jamieson
Proceedings of the National Academy of Sciences 2000
Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury
DM Toivola, NO Ku, N Ghori, AW Lowe, SA Michie, MB Omary
Experimental Cell Research 2000
Cerulein upregulates ICAM-1 in pancreatic acinar cells, which mediates neutrophil adhesion to these cells
V Zaninovic, AS Gukovskaya, I Gukovsky, M Mouria, SJ Pandol
AJP Gastrointestinal and Liver Physiology 2000
Regulation of adherens junction protein p120 ctn by 10 nM CCK precedes actin breakdown in rat pancreatic acini
J Leser, MF Beil, OA Musa, G Adler, MP Lutz
AJP Gastrointestinal and Liver Physiology 2000
Protein Tyrosine Dephosphorylation and the Maintenance of Cell Adhesions in the Pancreas
J Schnekenburger, J Mayerle, P Simon, W Domschke, MM Lerch
Annals of the New York Academy of Sciences 1999
Role of Actin in Regulated Exocytosis and Compensatory Membrane Retrieval: Insights from an Old Acquaintance
K Valentijn, JA Valentijn, JD Jamieson
Biochemical and Biophysical Research Communications 1999
Cholecystokinin-Induced Redistribution of Paxillin in Rat Pancreatic Acinar Cells
J Leser, H Lührs, MF Beil, G Adler, MP Lutz
Biochemical and Biophysical Research Communications 1999
Contributions of molecular motor enzymes to vesicle-based protein transport in gastrointestinal epithelial cells
MA McNiven, KJ Marlowe
Gastroenterology 1999
ETIOPATHOGENESIS OF ACUTE PANCREATITIS
S Karne, FS Gorelick
Surgical Clinics of North America 1999
The subapical actin cytoskeleton regulates secretion and membrane retrieval in pancreatic acinar cells
KM Valentijn, FD Gumkowski, JD Jamieson
Journal of cell science 1999
Cholecystokinin octapeptide inhibits Ca2+-dependent amylase secretion from permeabilized pancreatic acini by blocking the MgATP-dependent priming of exocytosis
PJ Padfield, N Panesar
Biochemical Journal 1998
Zymogen proteolysis within the pancreatic acinar cell is associated with cellular injury
T Grady, M MahMoud, T Otani, S Rhee, MM Lerch, FS Gorelick
AJP Gastrointestinal and Liver Physiology 1998
A Role for the p38 Mitogen-activated Protein Kinase/Hsp 27 Pathway in Cholecystokinin-induced Changes in the Actin Cytoskeleton in Rat Pancreatic Acini
C Schäfer, SE Ross, MJ Bragado, GE Groblewski, SA Ernst, JA Williams
The Journal of biological chemistry 1998
An ultrastructural study to investigate the effect of allopurinol on cerulein-induced damage to pancreatic acinar cells in rat
A Brunelli, G Scutti
International Journal of Gastrointestinal Cancer 1998
Disruption of Dictyostelium PI3K genes reduces [32P]phosphatidylinositol 3,4 bisphosphate and [32P]phosphatidylinositol trisphosphate levels, alters F-actin distribution and impairs pinocytosis
K Zhou, S Pandol, G Bokoch, AE Traynor-Kaplan
Journal of cell science 1998
The actin-myosin cytoskeleton mediates reversible agonist-induced membrane blebbing
RR Torgerson, MA McNiven
Journal of cell science 1998
Microtubules facilitate the stimulated secretion of beta-hexosaminidase in lacrimal acinar cells
SR da Costa, FA Yarber, L Zhang, M Sonee, SF Hamm-Alvarez
Journal of cell science 1998
Redifferentiation and apoptosis of pancreatic cells during acute pancreatitis
JL Iovanna
International Journal of Gastrointestinal Cancer 1996
Actin filament disassembly is a sufficient final trigger for exocytosis in nonexcitable cells
S Muallem, K Kwiatkowska, X Xu, HL Yin
The Journal of Cell Biology 1995
Effect of cerulein hyperstimulation on the paracellular barrier of rat exocrine pancreas
MB Fallon, FS Gorelick, JM Anderson, A Mennone, A Saluja, ML Steer
Gastroenterology 1995
Is an elevated concentration of acinar cytosolic free ionised calcium the trigger for acute pancreatitis?
JB Ward, SA Jenkins, R Sutton, OH Petersen
The Lancet 1995
Isolation of subcellular agonist-sensitive calcium stores from the pancreatic acinar cell
SJ Pandol, T Fitzsimmons, M Schoeffield-Payne, GW Carlile, WH Evans
Cell Calcium 1995
Cerulein-Induced pancreatic cysteine depletion: Prevention does not diminish acute pancreatitis in the mouse
BA Neuschwander-Tetri, M Barnidge, CG Janney
Gastroenterology 1994
Intermediate filaments in rat pancreatic acinar tumors, human ductal carcinomas, and other gastrointestinal malignancies
KH Herzig, M Altmannsberger, UR Fölsch
Gastroenterology 1994
Characterization of the three different states of the cholecystokinin (CCK) receptor in pancreatic acini
VD Talkad, RJ Patto, DC Metz, RJ Turner, KP Fortune, ST Bhat, JD Gardner
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1994
Facing the Pancreatic Dilemma
P Pederzoli, G Cavallini, C Bassi, M Falconi
1994
A newly recognized action of cholecystokinin on pancreatic acini-release of lactate dehydrogenase
ST Bhat, R Vinayek, RT Jensen, JD Gardner
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1993
Ökosystem Darm V
M Zeitz, WF Caspary, J Bockemühl, G Lux
1993
Pancreatic Disease
EC Kafka, MH Kalser
Postgraduate Medicine 1975

← Previous 1 2 3 4 Next →

Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts