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Referenced in 2 patents
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Research Article Free access | 10.1172/JCI114556

The complete digestion of human milk triacylglycerol in vitro requires gastric lipase, pancreatic colipase-dependent lipase, and bile salt-stimulated lipase.

S Bernbäck, L Bläckberg, and O Hernell

Department of Physiological Chemistry, University of Umeå, Sweden.

Find articles by Bernbäck, S. in: JCI | PubMed | Google Scholar

Department of Physiological Chemistry, University of Umeå, Sweden.

Find articles by Bläckberg, L. in: JCI | PubMed | Google Scholar

Department of Physiological Chemistry, University of Umeå, Sweden.

Find articles by Hernell, O. in: JCI | PubMed | Google Scholar

Published April 1, 1990 - More info

Published in Volume 85, Issue 4 on April 1, 1990
J Clin Invest. 1990;85(4):1221–1226. https://doi.org/10.1172/JCI114556.
© 1990 The American Society for Clinical Investigation
Published April 1, 1990 - Version history
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Abstract

Gastric lipase, pancreatic colipase-dependent lipase, and bile salt-stimulated lipase all have potential roles in digestion of human milk triacylglycerol. To reveal the function of each lipase, an in vitro study was carried out with purified lipases and cofactors, and with human milk as substrate. Conditions were chosen to resemble those of the physiologic environment in the gastrointestinal tract of breast-fed infants. Gastric lipase was unique in its ability to initiate hydrolysis of milk triacylglycerol. Activated bile salt-stimulated lipase could not on its own hydrolyze native milk fat globule triacylglycerol, whereas a limited hydrolysis by gastric lipase triggered hydrolysis by bile salt-stimulated lipase. Gastric lipase and colipase-dependent lipase, in combination, hydrolyzed about two thirds of total ester bonds, with monoacylglycerol and fatty acids being the end products. Addition of bile salt-stimulated lipase resulted in hydrolysis also of monoacylglycerol. When acting together with colipase-dependent lipase, bile salt-stimulated lipase contributed also to digestion of tri- and diacylglycerol. We conclude that digestion of human milk triacylglycerol depends on three lipases with unique, only partly overlapping, functions. Their concerted action results in complete digestion with free glycerol and fatty acids as final products.

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Referenced in 2 patents
61 readers on Mendeley
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