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Research Article Free access | 10.1172/JCI113967

Characterization of aldosterone-induced potassium secretion in rat distal colon.

J H Sweiry and H J Binder

Department of Internal Medicine, Yale University, New Haven, Connecticut 06510.

Find articles by Sweiry, J. in: PubMed | Google Scholar

Department of Internal Medicine, Yale University, New Haven, Connecticut 06510.

Find articles by Binder, H. in: PubMed | Google Scholar

Published March 1, 1989 - More info

Published in Volume 83, Issue 3 on March 1, 1989
J Clin Invest. 1989;83(3):844–851. https://doi.org/10.1172/JCI113967.
© 1989 The American Society for Clinical Investigation
Published March 1, 1989 - Version history
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Abstract

The role of apical and basolateral membranes in aldosterone-induced active potassium (K) secretion in rat distal colon was investigated by measuring mucosal-to-serosal (Jms) and serosal-to-mucosal (Jsm) 42K fluxes (mueq.h-1.cm-2) across isolated stripped mucosa under short-circuit conditions in normal and secondary-hyperaldosterone animals. In normal colons mucosal tetraethylammonium (TEA; 30 mM) or barium (Ba; 5 mM), but not cesium (Cs; 15 mM), reduced Jsm without affecting Jms. In aldosterone animals (a) net K secretion (-0.54 +/- 0.11) was converted to net K absorption (0.63 +/- 0.15) by mucosal TEA, which produced a marked reduction in Jsm (0.82 +/- 0.07) and an increase in Jms (0.35 +/- 0.07). In contrast mucosal Ba resulted in a relatively smaller reduction in JK(sm) without altering JK(ms), whereas mucosal Cs was ineffective; (b) serosal bumetanide or the removal of serosal Na or Cl markedly inhibited JK(sm and abolished net K secretion; and (c) serosal ouabain (1 mM) produced qualitatively similar effects to those of serosal bumetanide. These results demonstrate that (a) normal rat distal colon contains apical TEA- and Ba-sensitive K channels; (b) aldosterone induces TEA-sensitive and Ba-sensitive apical K channels; (c) aldosterone-induced K secretion requires both the Na,K-pump and Na-K-2Cl cotransport for K uptake across the basolateral membrane; and (d) alteration of any of these processes results in inhibition of aldosterone-induced active K secretion simultaneously with stimulation of K absorption.

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