Advertisement
Research Article Free access | 10.1172/JCI113751
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021.
Find articles by Gabrilove, J. in: JCI | PubMed | Google Scholar
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021.
Find articles by Jakubowski, A. in: JCI | PubMed | Google Scholar
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021.
Find articles by Fain, K. in: JCI | PubMed | Google Scholar
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021.
Find articles by Grous, J. in: JCI | PubMed | Google Scholar
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021.
Find articles by Scher, H. in: JCI | PubMed | Google Scholar
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021.
Find articles by Sternberg, C. in: JCI | PubMed | Google Scholar
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021.
Find articles by Yagoda, A. in: JCI | PubMed | Google Scholar
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021.
Find articles by Clarkson, B. in: JCI | PubMed | Google Scholar
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021.
Find articles by Bonilla, M. in: JCI | PubMed | Google Scholar
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021.
Find articles by Oettgen, H. in: JCI | PubMed | Google Scholar
Published October 1, 1988 - More info
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) was administered at a dose of 1-60 micrograms/kg of body weight to 22 patients with transitional cell carcinoma before chemotherapy as part of a Phase I/II study. In all patients, a specific dose-dependent increase in the absolute neutrophil count (ANC) of 1.8-12 fold was seen. In addition, this augmentation in the ANC was accompanied by an increase in leukocyte alkaline phosphatase, a marker of secondary granule formation. In six of eight patients analyzed, an increase in bone marrow myeloid to erythroid cell ratio was seen. Day 14 peripheral blood cell derived colony forming unit granulocyte macrophage were also increased by day 6 of rhG-CSF treatment. Circulating levels of eosinophils and basophils were unchanged; however, a 10-fold increase in monocytes was observed in patients treated at the highest doses. There was also a small increase in CD3+ lymphocytes that was not dose dependent. Hemoglobin, hematocrit, and platelet count remained near baseline throughout the period of rhG-CSF administration. These findings demonstrate that rhG-CSF is a potent stimulus for normal neutrophil proliferation and maturation.