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Research Article Free access | 10.1172/JCI113309

Close association between interleukin 2 receptor mRNA expression and human T cell leukemia/lymphoma virus type I viral RNA expression in short-term cultured leukemic cells from adult T cell leukemia patients.

H Umadome, T Uchiyama, T Hori, S Tamori, T Motoi, K Araki, and H Uchino

First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.

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First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.

Find articles by Uchiyama, T. in: JCI | PubMed | Google Scholar

First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.

Find articles by Hori, T. in: JCI | PubMed | Google Scholar

First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.

Find articles by Tamori, S. in: JCI | PubMed | Google Scholar

First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.

Find articles by Motoi, T. in: JCI | PubMed | Google Scholar

First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.

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First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.

Find articles by Uchino, H. in: JCI | PubMed | Google Scholar

Published January 1, 1988 - More info

Published in Volume 81, Issue 1 on January 1, 1988
J Clin Invest. 1988;81(1):52–61. https://doi.org/10.1172/JCI113309.
© 1988 The American Society for Clinical Investigation
Published January 1, 1988 - Version history
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Abstract

Human T cell leukemia/lymphoma (T-lymphotropic) virus type I (HTLV-I) infection has been considered to be closely associated with the leukemogenesis of adult T cell leukemia (ATL), in which interleukin 2 (IL-2) receptors are abnormally expressed. In this study, however, Southern blot analysis revealed no gross rearrangement or obvious amplification of the IL-2 receptor gene in ATL leukemic cells, indicating that abnormal IL-2 receptor expression in ATL is not due to the structural change of its gene. Hence, we studied the expression of the IL-2 receptor and HTLV-I at the RNA level during short-term cultures of leukemic cells from 9 ATL patients. Cytoplasmic dot hybridization and Northern hybridization revealed that fresh leukemic cells from seven of nine patients expressed a small amount of IL-2 receptor mRNA but HTLV-I RNA was undetectable in all cases. After cultures for up to 7 d, both IL-2 receptor mRNA and HTLV-I RNA (including pX message) expression concomitantly increased, whereas the amounts of other cellular genes, except for beta-actin, did not. The increases in their RNA expression were inhibited by early addition (within 12 h after the beginning of the culture) of cycloheximide, indicating that these increases are mediated by newly synthesized protein(s). These results strongly suggested that IL-2 receptor expression is closely associated with HTLV-I expression in leukemic cells from ATL patients.

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