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Research Article Free access | 10.1172/JCI112917

High frequency of autoantibodies bearing cross-reactive idiotopes among hybridomas using VH7183 genes prepared from normal and autoimmune murine strains.

B Bellon, A Manheimer-Lory, M Monestier, T Moran, A Dimitriu-Bona, F Alt, and C Bona

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Published April 1, 1987 - More info

Published in Volume 79, Issue 4 on April 1, 1987
J Clin Invest. 1987;79(4):1044–1053. https://doi.org/10.1172/JCI112917.
© 1987 The American Society for Clinical Investigation
Published April 1, 1987 - Version history
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Abstract

Hybridomas obtained by in vitro stimulation with lipopolysaccharides (LPS) of BALB/c, MRL/lpr, and NZB splenocytes were selected for expression of VH7183 by hybridization using slot blotting. Northern blot analysis showed that the majority of hybrids produce a full length message complementary to the VH7183 probe. The frequency of VH7183 hybridomas was significantly higher in NZB mice as compared with BALB/c mice. Using multiple binding assays, 60% of the total antibodies encoded by VH7183 were specific for self-epitopes. Finally, the vast majority express cross-reactive idiotypes borne by autoantibodies of various specificities.

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