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Article has an altmetric score of 3

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Referenced in 1 patents
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Research Article Free access | 10.1172/JCI112546

Localization of the binding site of tissue-type plasminogen activator to fibrin.

A Ichinose, K Takio, and K Fujikawa

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Published July 1, 1986 - More info

Published in Volume 78, Issue 1 on July 1, 1986
J Clin Invest. 1986;78(1):163–169. https://doi.org/10.1172/JCI112546.
© 1986 The American Society for Clinical Investigation
Published July 1, 1986 - Version history
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Abstract

Functionally active A and B chains were separated from a two-chain form of recombinant tissue-type plasminogen activator after mild reduction and alkylation. The A chain was found to be responsible for the binding to lysine-Sepharose or fibrin and the B chain contained the catalytic activity of tissue-type plasminogen activator. An extensive reduction of two-chain tissue-type plasminogen activator, however, destroyed both the binding and catalytic activities. A thermolytic fragment, Fr. 1, of tissue-type plasminogen activator that contained a growth factor and two kringle segments retained its lysine binding activity. Additional thermolytic cleavages in the kringle-2 segment of Fr. 1 caused a total loss of the binding activity. These results indicated that the binding site of tissue-type plasminogen activator to fibrin was located in the kringle-2 segment.

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Referenced in 1 patents
30 readers on Mendeley
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