Analysis of helper activity on pokeweed mitogen- and interleukin 2-driven immunoglobulin synthesis by neoplastic T4+ cells.

F Miedema; J W van Oostveen; F G Terpstra; A W van den Wall Bake; R Willemze; E A Rauws; R Bieger; M B van 't Veer; D Catovsky; C J Melief

doi:10.1172/JCI112219

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Published in Volume 76, Issue 6 on December 1, 1985
J Clin Invest. 1985;76(6):2139–2143. https://doi.org/10.1172/JCI112219.
© 1985 The American Society for Clinical Investigation

Published December 1, 1985 - Version history

The neoplastic T cells of a series of seven patients with chronic T-cell neoplasia were tested for helper activity on pokeweed mitogen (PWM)-induced and interleukin 2 (IL-2)-induced Ig synthesis. The neoplastic T cells of all patients had a T3+4+8-11+I1- phenotype but differed in expression of the 3A1 antigen. The neoplastic T cells of three patients had helper activity on both PWM- and IL-2-driven Ig synthesis, and in addition produced IL-2 in response to PWM stimulation. Two of these patients had hypergammaglobulinemia. In contrast, the neoplastic T cells in the remaining four patients did not produce IL-2 and did not support PWM-driven Ig synthesis. The T4+ cells of these four patients, however, provided excellent helper activity on IL-2-driven Ig synthesis. These findings emphasize the role of IL-2 in T cell-dependent Ig synthesis and clearly show that IL-2 production is required for helper activity in the PWM-driven system. It is concluded that the combined use of PWM- and IL-2-driven Ig synthesis systems allows separate analysis of IL-2 production and T-helper activity in health and disease.

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Analysis of helper activity on pokeweed mitogen- and interleukin 2-driven immunoglobulin synthesis by neoplastic T4+ cells.

F Miedema, J W van Oostveen, F G Terpstra, A W van den Wall Bake, R Willemze, E A Rauws, R Bieger, M B van 't Veer, D Catovsky, and C J Melief

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Analysis of helper activity on pokeweed mitogen- and interleukin 2-driven immunoglobulin synthesis by neoplastic T4+ cells.

F Miedema, J W van Oostveen, F G Terpstra, A W van den Wall Bake, R Willemze, E A Rauws, R Bieger, M B van 't Veer, D Catovsky, and C J Melief

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