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Research Article Free access | 10.1172/JCI112087

Purification of fetal hematopoietic progenitors and demonstration of recombinant multipotential colony-stimulating activity.

S G Emerson, C A Sieff, E A Wang, G G Wong, S C Clark, and D G Nathan

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Published September 1, 1985 - More info

Published in Volume 76, Issue 3 on September 1, 1985
J Clin Invest. 1985;76(3):1286–1290. https://doi.org/10.1172/JCI112087.
© 1985 The American Society for Clinical Investigation
Published September 1, 1985 - Version history
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Abstract

To facilitate the direct study of progenitor cell biology, we have developed a simple and efficient procedure based upon negative selection by panning to purify large numbers of committed erythroid and myeloid progenitors from human fetal liver. The nonadherent, panned cells constitute a highly enriched population of progenitor cells, containing 30.4 +/- 13.1% erythrocyte burst forming units (BFU-E), 5.5 +/- 1.9% granulocyte-macrophage colony forming units (CFU-GM), and 1.4 +/- 0.7% granulocyte-erythroid-macrophage-megakaryocyte colony forming units (CFU-GEMM) as assayed in methylcellulose cultures. These cells are morphologically immature blasts with prominent Golgi. This preparative method recovers 60-100% of the committed progenitors detectable in unfractionated fetal liver and yields 2-30 X 10(6) progenitors from each fetal liver sample, and thus provides sufficient numbers of enriched progenitors to allow direct biochemical and immunologic manipulation. Using this technique, a purified recombinant protein previously thought to have only granulocyte-macrophage colony stimulating activity (GM-CSA) is shown to have both burst promoting activity and multipotential colony stimulating activity. Progenitor purification by panning thus appears to be a simple, efficient method that should facilitate the direct study of committed hematopoietic progenitors and their differentiation.

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Referenced in 5 patents
18 readers on Mendeley
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