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Research Article Free access | 10.1172/JCI110748

Preferential binding of vasoactive intestinal polypeptide to basolateral membrane of rat and rabbit enterocytes.

K Dharmsathaphorn, V Harms, D J Yamashiro, R J Hughes, H J Binder, and E M Wright

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Published January 1, 1983 - More info

Published in Volume 71, Issue 1 on January 1, 1983
J Clin Invest. 1983;71(1):27–35. https://doi.org/10.1172/JCI110748.
© 1983 The American Society for Clinical Investigation
Published January 1, 1983 - Version history
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Abstract

Binding of radioiodinated vasoactive intestinal polypeptide (VIP) to intestinal cell membranes of the rabbit ileum and rat jejunum was investigated. Specific binding of 125I-labeled VIP could be demonstrated only on the basolateral membrane and not on the brush border membrane. This corresponded with the lack of an effect on ion transport when VIP was applied to the mucosal side of an in vitro preparation of rabbit ileum. VIP altered ion transport only when it was applied to the serosal side. The binding of 125I-VIP was specific and dependent upon incubation temperature. There was a close correlation between the potency of VIP for inhibition of 125I-VIP binding and that for increasing adenylate cyclase activity. These observations demonstrate that VIP receptors are located on the basolateral membrane.

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