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Usage Information

Circadian Rhythm of Intestinal Sucrase Activity in Rats: MECHANISM OF ENZYME CHANGE
Mark A. Kaufman, … , Helen A. Korsmo, Ward A. Olsen
Mark A. Kaufman, … , Helen A. Korsmo, Ward A. Olsen
Published May 1, 1980
Citation Information: J Clin Invest. 1980;65(5):1174-1181. https://doi.org/10.1172/JCI109772.
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Circadian Rhythm of Intestinal Sucrase Activity in Rats: MECHANISM OF ENZYME CHANGE

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Abstract

Past investigation has revealed that the circadian rhythm of intestinal sucrase activity in rats is primarily cued by the time of feeding. We examined the mechanism of the circadian rhythm by methods involving quantitative immunoprecipitation of sucrase-isomaltase protein and study of decay of radioactively labeled protein. Rats were placed on a controlled feeding regimen (1000-1500 h) and then sacrificed at 3-h intervals over a 24-h period. Immunotitration experiments indicated that the circadian rhythm was the result of changes in the absolute amount of sucrase-isomaltase protein present and not of changes in the enzyme's catalytic efficiency.

Authors

Mark A. Kaufman, Helen A. Korsmo, Ward A. Olsen

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