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Free access | 10.1172/JCI109324
Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510
Department of Medicine, Medical College of Virginia, Richmond, Virginia 23298
Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Department of Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
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Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510
Department of Medicine, Medical College of Virginia, Richmond, Virginia 23298
Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Department of Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Find articles by Walker, L. in: JCI | PubMed | Google Scholar
Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510
Department of Medicine, Medical College of Virginia, Richmond, Virginia 23298
Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Department of Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Find articles by Steere, A. in: JCI | PubMed | Google Scholar
Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510
Department of Medicine, Medical College of Virginia, Richmond, Virginia 23298
Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Department of Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
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Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510
Department of Medicine, Medical College of Virginia, Richmond, Virginia 23298
Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Department of Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
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Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510
Department of Medicine, Medical College of Virginia, Richmond, Virginia 23298
Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Department of Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
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Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510
Department of Medicine, Medical College of Virginia, Richmond, Virginia 23298
Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Department of Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Find articles by Ruddy, S. in: JCI | PubMed | Google Scholar
Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510
Department of Medicine, Medical College of Virginia, Richmond, Virginia 23298
Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Department of Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131
Find articles by Malawista, S. in: JCI | PubMed | Google Scholar
Published March 1, 1979 - More info
We have found immunoglobulin (Ig) G-containing material consistent with immune complexes in the sera of patients with Lyme arthritis. It was detected in 29 of 55 sera (55%) from 31 patients by at least one of three assays: 125I-C1q binding, C1q solid phase, or Raji cell. The presence of reactive material correlated with clinical aspects of disease activity; it was found early in the illness, was most prominent in sera from the sickest patients, was infrequent during remissions, and often fluctuated in parallel with changes in clinical status. The results in the two C1q assays showed a strong positive correlation (P<0.001). They were each elevated in 45% of the sera and were usually concordant (85%). In contrast, the Raji cell assay was less frequently positive and often discordant with the C1q assays. In sucrose density gradients, putative circulating immune complexes sedimented near 19S; they, too, were detected best by the two assays based on C1q binding. An additional 7S component was found in some sera by the 125I-C1q binding assay. Serum complement was often above the range of normal in patients with mild disease and normal in patients with severe disease but did not correlate significantly with levels of circulating immune complexes. IgM and IgG rheumatoid factors were not detectable.
These findings support a role for immune complexes in the pathogenesis of Lyme arthritis. Their measurement, by either the 125I-C1q binding assay or by the C1q solid phase assay, often provides a sensitive index of disease activity. Moreover, the complexes are likely sources of disease-related antigens for further study of this new disorder.