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Citations to this article

Pemphigus antibody interaction with human epidermal cells in culture.
J R Schiltz, … , B Michel, R Papay
J R Schiltz, … , B Michel, R Papay
Published October 1, 1978
Citation Information: J Clin Invest. 1978;62(4):778-788. https://doi.org/10.1172/JCI109189.
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Research Article

Pemphigus antibody interaction with human epidermal cells in culture.

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Abstract

The mechanism of pemphigus acantholysis has been studied with an in vitro system. Freshly prepared human skin epidermal cells were incubated in F-10 medium which contained the immunoglobulin G fraction from either pemphigus serum or normal human serum. During 18-h incubation periods, the pemphigus antibody became bound to the surface of the epidermal cells, caused the destruction of 75% of the viable cells as compared to only 14% in the normal immunoglobulin G controls (trypan blue exclusion), prevented the accumulation of newly synthesized proteins by nearly 60% as determined by radioactive tracer studies, and caused a dramatic shift in distribution of the newly synthesized proteins from an insoluble cell-associated fraction to an extracellular soluble fraction. These effects on the accumulation and partitioning of newly synthesized proteins were antibody concentration-dependent. Kinetic studies showed that at a fixed pemphigus antibody concentration the inhibition of protein accumulation preceded solubilization by about 1 h, at which time rapid solubilization of up to 70% of the insoluble cellular material occurred. Several lines of evidence suggested that this phenomenon was caused by enzymatic activity. Epidermal extracts solubilized a prepared substrate of radioactivity labeled insoluble epidermal cell material. This activity was heat labile and pH dependent, with pH optima ranging from 4.5 to 6.5. Enzymes with pH optima between 6 and 6.5 were recovered in the culture medium after a 2-day incubation of pure, intact epidermis with the pemphigus antibody. We proposed the following hypothesis to account for pemphigus acantholysis. The pemphigus antibody reacts with the epidermal cell surface and produces such a severe disturbance that the integrity of the cell surface is lost. As a result of these primary perturbations, the cell is killed and during the process, responds by release or activiation of soluble hydrolytic enzymes. This autolytic process results in the characteristic acantholysis of pemphigus.

Authors

J R Schiltz, B Michel, R Papay

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Total citations by year

Year: 2022 2020 2019 2013 2012 2011 2010 2008 2006 2005 2004 2002 2000 1999 1997 1996 1994 1992 1991 1989 1988 1987 1986 1985 1984 1983 1982 1981 1980 1979 Total
Citations: 1 1 1 1 1 2 3 2 1 2 1 1 1 1 2 1 1 1 2 5 3 1 1 7 5 7 3 7 4 2 71
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Citations to this article (71)

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H Koga, D Tsuruta, B Ohyama, N Ishii, T Hamada, C Ohata, M Furumura, T Hashimoto
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Deposition of the Membrane Attack Complex of Complement in Pemphigus Vulgaris and Pemphigus Foliaceus Skin
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Is the Ig Bound to Cytoid Bodies Antibody?
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