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Immunoinhibition of ristocetin-induced platelet aggregation.
R L Nachman, … , E A Jaffe, B B Weksler
R L Nachman, … , E A Jaffe, B B Weksler
Published January 1, 1977
Citation Information: J Clin Invest. 1977;59(1):143-148. https://doi.org/10.1172/JCI108612.
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Research Article

Immunoinhibition of ristocetin-induced platelet aggregation.

Abstract

Human platelets washed and fixed in paraformaldehyde aggregate in the presence of the antibiotic ristocetin and normal plasma. This aggregation response is abolished after digestion of the fixed platelets with chymotrypsin. Antisera to fixed washed platelets were produced in rabbits and absorbed with chymotrypsin-treated, fixed washed platelets. Monovalent Fab fragments obtained from the isolated gamma-globulin fractions of the antisera blocked ristocetin-induced aggregation of fixed washed platelets in buffer and normal platelets in platelet-rich plasma. By double-antibody immunoprecipitation, it was shown that the antibody which blocked the ristocetin reaction interacted with a platelet membrane surface protein of mol wt 155,000. The results suggest that the glycoprotein I complex on the surface of the human platelet mediates ristocetin-induced von Willebrand factor-dependent platelet aggregation.

Authors

R L Nachman, E A Jaffe, B B Weksler

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