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Research Article Free access | 10.1172/JCI107980
Find articles by Hohn, D. in: JCI | PubMed | Google Scholar
Find articles by Lehrer, R. in: JCI | PubMed | Google Scholar
Published April 1, 1975 - More info
We measured the cyanide-insensitive pyridine nucleotide oxidase activity of fractionated resting and phagocytic neutrophils from 11 normal donors, 1 patient with hereditary deficiency of myeloperoxidase, and 7 patients with X-linked chronic granulomatous disease (CGD). When measured under optimal conditions (at pH 5.5 and in the presence of 0.5 mM Mn++), NADPH oxidase activity increased fourfold with phagocytosis and was six-fold higher than with NADH. Phagocytic neutrophils from patients with CGD were markedly deficient in NADPH oxidase activity.
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