Advertisement
Research Article Free access | 10.1172/JCI107736
Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biometry, Emory University School of Medicine, Atlanta, Georgia 30322
Clinical Research Facility, Emory University Hospital, Atlanta, Georgia 30322
Find articles by Rudman, D. in: JCI | PubMed | Google Scholar
Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biometry, Emory University School of Medicine, Atlanta, Georgia 30322
Clinical Research Facility, Emory University Hospital, Atlanta, Georgia 30322
Find articles by Chawla, R. in: JCI | PubMed | Google Scholar
Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biometry, Emory University School of Medicine, Atlanta, Georgia 30322
Clinical Research Facility, Emory University Hospital, Atlanta, Georgia 30322
Find articles by Del Rio, A. in: JCI | PubMed | Google Scholar
Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biometry, Emory University School of Medicine, Atlanta, Georgia 30322
Clinical Research Facility, Emory University Hospital, Atlanta, Georgia 30322
Find articles by Hollins, B. in: JCI | PubMed | Google Scholar
Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biometry, Emory University School of Medicine, Atlanta, Georgia 30322
Clinical Research Facility, Emory University Hospital, Atlanta, Georgia 30322
Find articles by Hall, E. in: JCI | PubMed | Google Scholar
Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322
Department of Biometry, Emory University School of Medicine, Atlanta, Georgia 30322
Clinical Research Facility, Emory University Hospital, Atlanta, Georgia 30322
Find articles by Conn, J. in: JCI | PubMed | Google Scholar
Published July 1, 1974 - More info
22 nonneoplastic, noninflammatory effusions (cirrhosis and congestive heart failure), 12 non-neoplastic inflammatory effusions (tuberculosis, lupus erythematosus, rheumatoid arthritis, and idiopathic pleuropericarditis), and 58 neoplastic effusions (cancer of lung, breast, ovary, and pancreas, and lymphoma) were analyzed by radial immunodiffusion for orosomucoid concentration. The average concentration ±SE was 35±4, 65±17, and 130±13 mg/100 ml in the three types of effusion, respectively. By gel filtration and ion exchange chromatography, orosomucoid was isolated from 12 nonmalignant and 14 malignant fluids. The orosomucoid preparations reacted as single components in acrylamide gel electrophoresis at pH 9.0, and in immunodiffusion and immunoelectrophoresis against antisera to human serum and to human plasma orosomucoid. In radial immunodiffusion, the slope of the line relating concentration to the square of the diameter of the precipitate area was identical for orosomucoid isolated from normal human plasma and from nonneoplastic effusions, but was subnormal for orosomucoid isolated from neoplastic fluids. All orosomucoid preparations had normal amino acid composition. Orosomucoid from the nonmalignant effusions had normal carbohydrate content. 11 of 14 samples of orosomucoid isolated from neoplastic fluids had abnormalities in carbohydrate composition, consisting of subnormal content of sialic acid (11 of 14), hexose (10 of 14), and hexosamine (3 of 14), and abnormally high content of hexosamine (4 of 14).
Discriminant analysis showed that concentration of orosomucoid distinguished between neoplastic and nonneoplastic noninflammatory effusions more effectively than concentration of total protein, albumin, α1, α2, β, or γ-globulin.
Images.